A Novel Strategy for Creating Tissue-Engineered Biomimetic Blood Vessels Using 3D Bioprinting Technology

In this work, a novel strategy was developed to fabricate prevascularized cell-layer blood vessels in thick tissues and small-diameter blood vessel substitutes using three-dimensional (3D) bioprinting technology. These thick vascularized tissues were comprised of cells, a decellularized extracellula...

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Main Authors: Yuanyuan Xu, Yingying Hu, Changyong Liu, Hongyi Yao, Boxun Liu, Shengli Mi
Format: Article
Language:English
Published: MDPI AG 2018-09-01
Series:Materials
Subjects:
Online Access:http://www.mdpi.com/1996-1944/11/9/1581
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spelling doaj-0a9364cf478044deaf6e8e3562c5a2bb2020-11-24T21:47:55ZengMDPI AGMaterials1996-19442018-09-01119158110.3390/ma11091581ma11091581A Novel Strategy for Creating Tissue-Engineered Biomimetic Blood Vessels Using 3D Bioprinting TechnologyYuanyuan Xu0Yingying Hu1Changyong Liu2Hongyi Yao3Boxun Liu4Shengli Mi5Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, ChinaBiomanufacturing Engineering Laboratory, Advanced Manufacturing Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, ChinaAdditive Manufacturing Research Institute, College of Mechatronics and Control Engineering, Shenzhen University, Shenzhen 518060, ChinaBiomanufacturing Engineering Laboratory, Advanced Manufacturing Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, ChinaDepartment of Precision Medicine and Healthcare, Tsinghua-Berkeley Shenzhen Institute, Shenzhen 518055, ChinaBiomanufacturing Engineering Laboratory, Advanced Manufacturing Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, ChinaIn this work, a novel strategy was developed to fabricate prevascularized cell-layer blood vessels in thick tissues and small-diameter blood vessel substitutes using three-dimensional (3D) bioprinting technology. These thick vascularized tissues were comprised of cells, a decellularized extracellular matrix (dECM), and a vasculature of multilevel sizes and multibranch architectures. Pluronic F127 (PF 127) was used as a sacrificial material for the formation of the vasculature through a multi-nozzle 3D bioprinting system. After printing, Pluronic F127 was removed to obtain multilevel hollow channels for the attachment of human umbilical vein endothelial cells (HUVECs). To reconstruct functional small-diameter blood vessel substitutes, a supporting scaffold (SE1700) with a double-layer circular structure was first bioprinted. Human aortic vascular smooth muscle cells (HA-VSMCs), HUVECs, and human dermal fibroblasts–neonatal (HDF-n) were separately used to form the media, intima, and adventitia through perfusion into the corresponding location of the supporting scaffold. In particular, the dECM was used as the matrix of the small-diameter blood vessel substitutes. After culture in vitro for 48 h, fluorescent images revealed that cells maintained their viability and that the samples maintained structural integrity. In addition, we analyzed the mechanical properties of the printed scaffold and found that its elastic modulus approximated that of the natural aorta. These findings demonstrate the feasibility of fabricating different kinds of vessels to imitate the structure and function of the human vascular system using 3D bioprinting technology.http://www.mdpi.com/1996-1944/11/9/15813D bioprintingvascularized tissuessmall-diameter blood vesselsbiomimetic modelingdECM
collection DOAJ
language English
format Article
sources DOAJ
author Yuanyuan Xu
Yingying Hu
Changyong Liu
Hongyi Yao
Boxun Liu
Shengli Mi
spellingShingle Yuanyuan Xu
Yingying Hu
Changyong Liu
Hongyi Yao
Boxun Liu
Shengli Mi
A Novel Strategy for Creating Tissue-Engineered Biomimetic Blood Vessels Using 3D Bioprinting Technology
Materials
3D bioprinting
vascularized tissues
small-diameter blood vessels
biomimetic modeling
dECM
author_facet Yuanyuan Xu
Yingying Hu
Changyong Liu
Hongyi Yao
Boxun Liu
Shengli Mi
author_sort Yuanyuan Xu
title A Novel Strategy for Creating Tissue-Engineered Biomimetic Blood Vessels Using 3D Bioprinting Technology
title_short A Novel Strategy for Creating Tissue-Engineered Biomimetic Blood Vessels Using 3D Bioprinting Technology
title_full A Novel Strategy for Creating Tissue-Engineered Biomimetic Blood Vessels Using 3D Bioprinting Technology
title_fullStr A Novel Strategy for Creating Tissue-Engineered Biomimetic Blood Vessels Using 3D Bioprinting Technology
title_full_unstemmed A Novel Strategy for Creating Tissue-Engineered Biomimetic Blood Vessels Using 3D Bioprinting Technology
title_sort novel strategy for creating tissue-engineered biomimetic blood vessels using 3d bioprinting technology
publisher MDPI AG
series Materials
issn 1996-1944
publishDate 2018-09-01
description In this work, a novel strategy was developed to fabricate prevascularized cell-layer blood vessels in thick tissues and small-diameter blood vessel substitutes using three-dimensional (3D) bioprinting technology. These thick vascularized tissues were comprised of cells, a decellularized extracellular matrix (dECM), and a vasculature of multilevel sizes and multibranch architectures. Pluronic F127 (PF 127) was used as a sacrificial material for the formation of the vasculature through a multi-nozzle 3D bioprinting system. After printing, Pluronic F127 was removed to obtain multilevel hollow channels for the attachment of human umbilical vein endothelial cells (HUVECs). To reconstruct functional small-diameter blood vessel substitutes, a supporting scaffold (SE1700) with a double-layer circular structure was first bioprinted. Human aortic vascular smooth muscle cells (HA-VSMCs), HUVECs, and human dermal fibroblasts–neonatal (HDF-n) were separately used to form the media, intima, and adventitia through perfusion into the corresponding location of the supporting scaffold. In particular, the dECM was used as the matrix of the small-diameter blood vessel substitutes. After culture in vitro for 48 h, fluorescent images revealed that cells maintained their viability and that the samples maintained structural integrity. In addition, we analyzed the mechanical properties of the printed scaffold and found that its elastic modulus approximated that of the natural aorta. These findings demonstrate the feasibility of fabricating different kinds of vessels to imitate the structure and function of the human vascular system using 3D bioprinting technology.
topic 3D bioprinting
vascularized tissues
small-diameter blood vessels
biomimetic modeling
dECM
url http://www.mdpi.com/1996-1944/11/9/1581
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