The pelvis urinary microbiome in patients with kidney stones and clinical associations

• Main Authors: Fengping Liu, Nan Zhang, Yunhong Wu, Peng Jiang, Tingting Jiang, Yang Wang, Yuwei Zhang, Qixiao Zhai, Yeqing Zou, Ninghan Feng
• Format: Article
• Language: English
• Published: BMC 2020-11-01
• Series: BMC Microbiology
• Subjects: Bladder urinary microbiome; Blood microbiome; Kidney pelvis urinary microbiome; Kidney stone; Kidney function
• Online Access: http://link.springer.com/article/10.1186/s12866-020-01992-4

Abstract Background The long-held notion that, without urinary tract or circulatory infection, bladder urine and blood are sterile biofluids has been disproven. There have been no previous reports on the kidney pelvis urinary microbiome after bladder disinfection in kidney stone patients. This study aimed to determine whether a kidney pelvis urinary microbiome is present after eliminating the influence of the bladder urinary microbiome, whether the microbiome composition is different in patients with stone kidney pelvis (SKP) and non-stone kidney pelvis (NSKP), and the correlation between SKP and patient clinical characteristics. Results Comparisons of bacterial diversity and community structure exhibited that urine in bladder was similar to SKP and NSKP. However, the comparisons showed that urine samples were different from blood. The most common operational taxonomic units were shared by all three types of urine samples. Corynebacterium was significantly higher in SKP compared to NSKP. Several bacteria were associated with patient characteristics, including Lactobacillus, which was positively correlated with fasting blood glucose, and Prevotella was negatively correlated with BMI. Lactobacillus was significantly higher in SKP compared to blood but not in NSKP compared to blood. Conclusions The composition of the kidney pelvis urinary microbiome after disinfection of the bladder and its similarity to the bladder microbiome indicate that bladder urine can be used to replace kidney pelvis urine in microbiome research. Additionally, the comparison of SKP and NSKP and clinical associations suggest that the occurrence of kidney stones is responsible for the SKP urinary microbiome.