Protective effect of resolvin D1 pretreatment in a rat model of hepatic ischemia-reperfusion injury and its mechanism

• Main Author: WANG Yangyang
• Format: Article
• Language: zho
• Published: Editorial Department of Journal of Clinical Hepatology 2021-06-01
• Series: Linchuang Gandanbing Zazhi
• Online Access: http://www.lcgdbzz.org/cn/article/doi/10.3969/j.issn.1001-5256.2021.06.030

ObjectiveTo investigate the protective effect of resolvin D1 (RvD1) in a rat model of hepatic ischemia/reperfusion (IR) injury and its association with heme oxygenase-1 (HO-1). MethodsA total of 36 Sprague-Dawley rats were randomly divided into sham-operation (sham)+phosphate-buffered saline (PBS) group, sham+high-dose RvD1 (10 μg/kg) group, IR+PBS group, IR+low-dose RvD1 (2 μg/kg) group, IR+middle-dose RvD1 (5 μg/kg) group, and IR+high-dose RvD1 (10 μg/kg) group, with 6 rats in each group. RvD1 were intraperitoneally injected at 1 hour before ischemia. A biochemical analyzer was used to measure the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST); ELISA was used to measure the plasma levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-8 (IL-8); HE staining was used to observe the histological changes of the liver; Western blot was used to measure the change in HO-1 in liver tissue. A one-way analysis of variance was used for comparison of continuous data between multiple groups, and the least significant difference t-test was used for further comparison between two groups. ResultsCompared with the IR+PBS group, the IR+middle-dose RvD1 group and the IR+high-dose RvD1 group had significant reductions in the levels of ALT, AST, and the inflammatory factors TNF-α, IL-6, and IL-8 (all P＜0.05), and there were no significant differences between the IR+middle-dose RvD1 group and the IR+high-dose RvD1 group (all P＞0.05). Western blot showed that compared with the IR+PBS group, the IR+middle-dose RvD1 group and the IR+high-dose RvD1 group had a significant increase in the protein expression of HO-1 in the liver (P＜0.05). HE staining showed that compared with the IR+PBS group, the IR+middle-dose RvD1 group and the IR+high-dose RvD1 group had cell swelling and disordered hepatic cords, without massive necrosis. Conclusion RvD1 exerts a protective effect against hepatic IR injury in rats by increasing the expression of HO-1 and reducing the levels of inflammatory factors (TNF-α, IL-6, and IL-8) and aminotransferases (ALT and AST).