RESEARCHES REGARDING THE INFLUENCE OF RECOVERY MEDIA ON THE IN VITRO DEVELOPMENT CAPACITY OF THE PREIMPLANTATIONAL MOUSE EMBRYO

• Main Authors: ADA CEAN, N. PACALA, ALEXANDRA BOLEMAN
• Format: Article
• Language: English
• Published: Agroprint Timisoara 2009-05-01
• Series: Scientific Papers Animal Science and Biotechnologies
• Subjects: embryos; recovery media M2; PBS-0; 4%BSA; hatching rate
• Online Access: http://usab-tm.ro/fileadmin/fzb/simpozion%202009/Volumul1/biotehnologii/Cean%201.pdf

Phosphate Bufered Saline with 0.4% BSA and M2 medium are one of the most common media used in embryorecovery. The aim of our paper was to investigate if the recovery media used for the recovery of the mouseembryo is influencing in vitro developmental capacity. As biological material we used 10 used were mousefemales, age 2 months superovulated with 5UI PMSG (Pregnant Mare Serum Gonadotropine) and 5 UI hCG(human Corionic Gonadotropine). The embryos used were recovered, by oviduct flushing, at 24 hours from theidentification of the vaginal plug. The majority of the embryos (78.3%) were in two cells stage. A total of 123, 2cells embryos were cultivated in M16 medium. The evolution of the embryos was examined at 24, 48 and 72hours interval. The proportion of hatched blastocyst was higher at the embryos recovered with M2 (53.7%)compared with the embryos recovered with PBS 0.4% BSA. The difference is statistically very significant(p<0.001). Embryos recovered in M2 media have a higher in vitro developmental capacity compared with theembryos recovered in PBS media supplemented with 0,4% BSA, possibly because of the sodium bicarbonate andlactate used in M2 media for pH regulation.