C-terminal fluorescent labeling impairs functionality of DNA mismatch repair proteins.

The human DNA mismatch repair (MMR) process is crucial to maintain the integrity of the genome and requires many different proteins which interact perfectly and coordinated. Germline mutations in MMR genes are responsible for the development of the hereditary form of colorectal cancer called Lynch s...

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Main Authors: Angela Brieger, Guido Plotz, Inga Hinrichsen, Sandra Passmann, Ronja Adam, Stefan Zeuzem
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3279419?pdf=render
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spelling doaj-0a50d9902ed44f30b106ac0620341b822020-11-25T01:38:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0172e3186310.1371/journal.pone.0031863C-terminal fluorescent labeling impairs functionality of DNA mismatch repair proteins.Angela BriegerGuido PlotzInga HinrichsenSandra PassmannRonja AdamStefan ZeuzemThe human DNA mismatch repair (MMR) process is crucial to maintain the integrity of the genome and requires many different proteins which interact perfectly and coordinated. Germline mutations in MMR genes are responsible for the development of the hereditary form of colorectal cancer called Lynch syndrome. Various mutations mainly in two MMR proteins, MLH1 and MSH2, have been identified so far, whereas 55% are detected within MLH1, the essential component of the heterodimer MutLα (MLH1 and PMS2). Most of those MLH1 variants are pathogenic but the relevance of missense mutations often remains unclear. Many different recombinant systems are applied to filter out disease-associated proteins whereby fluorescent tagged proteins are frequently used. However, dye labeling might have deleterious effects on MutLα's functionality. Therefore, we analyzed the consequences of N- and C-terminal fluorescent labeling on expression level, cellular localization and MMR activity of MutLα. Besides significant influence of GFP- or Red-fusion on protein expression we detected incorrect shuttling of single expressed C-terminal GFP-tagged PMS2 into the nucleus and found that C-terminal dye labeling impaired MMR function of MutLα. In contrast, N-terminal tagged MutLαs retained correct functionality and can be recommended both for the analysis of cellular localization and MMR efficiency.http://europepmc.org/articles/PMC3279419?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Angela Brieger
Guido Plotz
Inga Hinrichsen
Sandra Passmann
Ronja Adam
Stefan Zeuzem
spellingShingle Angela Brieger
Guido Plotz
Inga Hinrichsen
Sandra Passmann
Ronja Adam
Stefan Zeuzem
C-terminal fluorescent labeling impairs functionality of DNA mismatch repair proteins.
PLoS ONE
author_facet Angela Brieger
Guido Plotz
Inga Hinrichsen
Sandra Passmann
Ronja Adam
Stefan Zeuzem
author_sort Angela Brieger
title C-terminal fluorescent labeling impairs functionality of DNA mismatch repair proteins.
title_short C-terminal fluorescent labeling impairs functionality of DNA mismatch repair proteins.
title_full C-terminal fluorescent labeling impairs functionality of DNA mismatch repair proteins.
title_fullStr C-terminal fluorescent labeling impairs functionality of DNA mismatch repair proteins.
title_full_unstemmed C-terminal fluorescent labeling impairs functionality of DNA mismatch repair proteins.
title_sort c-terminal fluorescent labeling impairs functionality of dna mismatch repair proteins.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2012-01-01
description The human DNA mismatch repair (MMR) process is crucial to maintain the integrity of the genome and requires many different proteins which interact perfectly and coordinated. Germline mutations in MMR genes are responsible for the development of the hereditary form of colorectal cancer called Lynch syndrome. Various mutations mainly in two MMR proteins, MLH1 and MSH2, have been identified so far, whereas 55% are detected within MLH1, the essential component of the heterodimer MutLα (MLH1 and PMS2). Most of those MLH1 variants are pathogenic but the relevance of missense mutations often remains unclear. Many different recombinant systems are applied to filter out disease-associated proteins whereby fluorescent tagged proteins are frequently used. However, dye labeling might have deleterious effects on MutLα's functionality. Therefore, we analyzed the consequences of N- and C-terminal fluorescent labeling on expression level, cellular localization and MMR activity of MutLα. Besides significant influence of GFP- or Red-fusion on protein expression we detected incorrect shuttling of single expressed C-terminal GFP-tagged PMS2 into the nucleus and found that C-terminal dye labeling impaired MMR function of MutLα. In contrast, N-terminal tagged MutLαs retained correct functionality and can be recommended both for the analysis of cellular localization and MMR efficiency.
url http://europepmc.org/articles/PMC3279419?pdf=render
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