Diphenyleneiodonium, an inhibitor of NOXes and DUOXes, is also an iodide-specific transporter
NADPH oxidases (NOXes) and dual oxidases (DUOXes) generate O2.− and H2O2. Diphenyleneiodonium (DPI) inhibits the activity of these enzymes and is often used as a specific inhibitor. It is shown here that DPI, at concentrations similar to those which inhibit the generation of O2 derivatives, activate...
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Wiley
2014-01-01
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| Series: | FEBS Open Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211546313000740 |
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doaj-0a4fe718794c4b988396743248c2d9d52020-11-25T03:29:32ZengWileyFEBS Open Bio2211-54632014-01-014C555910.1016/j.fob.2013.11.007Diphenyleneiodonium, an inhibitor of NOXes and DUOXes, is also an iodide-specific transporterC. Massart0N. Giusti1R. Beauwens2J.E. Dumont3F. Miot4J. Van Sande5IRIBHM, School of Medicine, ULB, Université Libre de Bruxelles, Campus Erasme, Route de Lennik 808, BelgiumIRIBHM, School of Medicine, ULB, Université Libre de Bruxelles, Campus Erasme, Route de Lennik 808, BelgiumLaboratory of Physiology, School of Medicine, ULB, Université Libre de Bruxelles, Campus Erasme, Route de Lennik 808, BelgiumIRIBHM, School of Medicine, ULB, Université Libre de Bruxelles, Campus Erasme, Route de Lennik 808, BelgiumIRIBHM, School of Medicine, ULB, Université Libre de Bruxelles, Campus Erasme, Route de Lennik 808, BelgiumIRIBHM, School of Medicine, ULB, Université Libre de Bruxelles, Campus Erasme, Route de Lennik 808, BelgiumNADPH oxidases (NOXes) and dual oxidases (DUOXes) generate O2.− and H2O2. Diphenyleneiodonium (DPI) inhibits the activity of these enzymes and is often used as a specific inhibitor. It is shown here that DPI, at concentrations similar to those which inhibit the generation of O2 derivatives, activated the efflux of radioiodide but not of its analog 99mTcO4− nor of the K+ cation mimic 86Rb+ in thyroid cells, in the PCCl3 rat thyroid cell line and in COS cell lines expressing the iodide transporter NIS. Effects obtained with DPI, especially in thyroid cells, should therefore be interpreted with caution.http://www.sciencedirect.com/science/article/pii/S2211546313000740DiphenyleneiodoniumO2 radical generationNOXDUOXIodide effluxThyroid |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
C. Massart N. Giusti R. Beauwens J.E. Dumont F. Miot J. Van Sande |
| spellingShingle |
C. Massart N. Giusti R. Beauwens J.E. Dumont F. Miot J. Van Sande Diphenyleneiodonium, an inhibitor of NOXes and DUOXes, is also an iodide-specific transporter FEBS Open Bio Diphenyleneiodonium O2 radical generation NOX DUOX Iodide efflux Thyroid |
| author_facet |
C. Massart N. Giusti R. Beauwens J.E. Dumont F. Miot J. Van Sande |
| author_sort |
C. Massart |
| title |
Diphenyleneiodonium, an inhibitor of NOXes and DUOXes, is also an iodide-specific transporter |
| title_short |
Diphenyleneiodonium, an inhibitor of NOXes and DUOXes, is also an iodide-specific transporter |
| title_full |
Diphenyleneiodonium, an inhibitor of NOXes and DUOXes, is also an iodide-specific transporter |
| title_fullStr |
Diphenyleneiodonium, an inhibitor of NOXes and DUOXes, is also an iodide-specific transporter |
| title_full_unstemmed |
Diphenyleneiodonium, an inhibitor of NOXes and DUOXes, is also an iodide-specific transporter |
| title_sort |
diphenyleneiodonium, an inhibitor of noxes and duoxes, is also an iodide-specific transporter |
| publisher |
Wiley |
| series |
FEBS Open Bio |
| issn |
2211-5463 |
| publishDate |
2014-01-01 |
| description |
NADPH oxidases (NOXes) and dual oxidases (DUOXes) generate O2.− and H2O2. Diphenyleneiodonium (DPI) inhibits the activity of these enzymes and is often used as a specific inhibitor. It is shown here that DPI, at concentrations similar to those which inhibit the generation of O2 derivatives, activated the efflux of radioiodide but not of its analog 99mTcO4− nor of the K+ cation mimic 86Rb+ in thyroid cells, in the PCCl3 rat thyroid cell line and in COS cell lines expressing the iodide transporter NIS. Effects obtained with DPI, especially in thyroid cells, should therefore be interpreted with caution. |
| topic |
Diphenyleneiodonium O2 radical generation NOX DUOX Iodide efflux Thyroid |
| url |
http://www.sciencedirect.com/science/article/pii/S2211546313000740 |
| work_keys_str_mv |
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