Implantation Serine Proteinases heterodimerize and are critical in hatching and implantation

• Main Authors: Meng Guoliang, Irwin Jackie, Tang Lin, Liu Shiying, Sharma Navneet, Rancourt Derrick E
• Format: Article
• Language: English
• Published: BMC 2006-12-01
• Series: BMC Developmental Biology
• Online Access: http://www.biomedcentral.com/1471-213X/6/61

<p>Abstract</p> <p>Background</p> <p>We have recently reported the expression of murine Implantation Serine Proteinase genes in pre-implantation embryos (ISP1) and uterus (ISP1 and ISP2). These proteinases belong to the S1 proteinase family and are similar to mast cell tryptases, which function as multimers.</p> <p>Results</p> <p>Here, we report the purification and initial characterization of ISP1 and 2 with respect to their physico-chemical properties and physiological function. In addition to being co-expressed in uterus, we show that ISP1 and ISP2 are also co-expressed in the pre-implantation embryo. Together, they form a heterodimer with an approximate molecular weight of 63 kD. This complex is the active form of the enzyme, which we have further characterized as being trypsin-like, based on substrate and inhibitor specificities. In addition to having a role in embryo hatching and outgrowth, we demonstrate that ISP enzyme is localized to the site of embryo invasion during implantation and that its activity is important for successful implantation <it>in vivo</it>.</p> <p>Conclusion</p> <p>On the basis of similarities in structural, chemical, and functional properties, we suggest that this ISP enzyme complex represents the classical hatching enzyme, strypsin. Our results demonstrate a critical role for ISP in embryo hatching and implantation.</p>