Effect natural immunomodulators influence of cellular immunity indices and cortisol level in rat’s blood at pre-slaughter stress

• Main Author: S. S. Grabovskyi
• Format: Article
• Language: English
• Published: Львівський національний університет імені Івана Франка 2014-04-01
• Series: Біологічні студії
• Subjects: rats; for pre-slaughter stress; spleen; echinacea and schizandra chinensis; extracts; growing grain; t- and b-lymphocytes; cortisol
• Online Access: http://publications.lnu.edu.ua/journals/index.php/biology/article/view/157
• ISSN: 1996-4536; 2311-0783

Cellular immunity indices and cortisol level in rat’s blood after using of plant and animal origin immunomodulator were studied. Relative amount of T- and B-lymphocytes and their populations in the reaction of spontaneous rоsetting with the ram erythrocytes in was determined blood. Differentiated count of rоsetting lymphocyte with the different functional activity degree was conducted. Aerosol introduction of spleen extract to the rats feed strengthens the cells receptor apparatus of rat’s blood cells: increases the amount of total T-cell low-density receptors, T-cell active and T-cell of “zero” helpers, compared to control T- and B-lymphocytes number and functional activity T- and B-cell immunity was stimulated after additional supplementary of rat’s diet with experimental natural immunomodulators. Cortisol level in rat’s blood was reliable lower compared to control that caused decreasing pre-slaughter stress. Spleen extract polyamines as the immunomodulators and antistressors most effectively influenced some T- и B-cell immunity indices and cortisol level in rat’s blood before slaughter. The results obtained in model experiment can be used in studies of cell immunity indices and stress hormones, such as cortisol, on farm animals for organism resistance increasing and correction of their pre-slaughter stress.