PCR IN TRAUMATOLOGY AND ORTHOPAEDICS: METHOD DESCRIPTION AND APPLICABILITY

• Main Authors: E. M. Polyakova, S. A. Bozhkova
• Format: Article
• Language: Russian
• Published: Vreden Russian Research Institute of Traumatology and Orthopedics 2016-09-01
• Series: Travmatologiâ i Ortopediâ Rossii
• Subjects: полимеразная цепная реакция; имплантат-ассоциированная инфекция; антибиотикорезистентность; биопленки; polymerase chain reaction; implant-associated infection; antibiotic resistance; biofilms
• Online Access: https://journal.rniito.org/jour/article/view/84
• ISSN: 2311-2905; 2542-0933

Review brief presents description of polymerase chain reaction method (PCR) and its most common variants. Three PCR-based lines of research, carried out in the traumatology and orthopaedics, include identifying a causative agents of the implant-associated infection after orthopaedic surgery; detection of antibiotic resistance genes and biofilm forming genes. It was shown that PCR can be used as additional method for detection of genetic disorders, significant for traumatology and orthopaedics, and for investigation of cartilage and bone regeneration.