Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing

Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing

Heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing were utilised to genotype human parvovirus B19 samples from Brazil and Paraguay. Ninety-seven serum samples were collected from individuals presenting with abortion or erythema infectiosum, arthropathies...

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Main Authors: Marcos César Lima de Mendonça, Ana Maria de Amorim Ferreira, Marta Gonçalves Matos dos Santos, Elva Cristina Oviedo, Maria Sônia Dal Bello, Marilda Mendonça Siqueira, Juan Manuel Piñeiro Maceira, Maria Genoveva von Hubinger, José Nelson dos Santos Silva Couceiro
Format: Article
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2011-06-01
Series:Memórias do Instituto Oswaldo Cruz.
Subjects:
parvovirus B19
nucleotide sequencing
genotyping
HMA
SSCP
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400019&lng=en&tlng=en
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spelling doaj-0961792dcc9341649da80bb9a06d92cf2020-11-25T01:35:10ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.1678-80602011-06-01106450250410.1590/S0074-02762011000400019S0074-02762011000400019Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencingMarcos César Lima de Mendonça0Ana Maria de Amorim Ferreira1Marta Gonçalves Matos dos Santos2Elva Cristina Oviedo3Maria Sônia Dal Bello4Marilda Mendonça Siqueira5Juan Manuel Piñeiro Maceira6Maria Genoveva von Hubinger7José Nelson dos Santos Silva Couceiro8Universidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroMinisterio de Salud PúblicaUniversidade de Passo FundoFundação Oswaldo CruzUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroHeteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing were utilised to genotype human parvovirus B19 samples from Brazil and Paraguay. Ninety-seven serum samples were collected from individuals presenting with abortion or erythema infectiosum, arthropathies, severe anaemia and transient aplastic crisis; two additional skin samples were collected by biopsy. After the procedure, all clinical samples were classified as genotype 1.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400019&lng=en&tlng=enparvovirus B19nucleotide sequencinggenotypingHMASSCP
collection DOAJ
language English
format Article
sources DOAJ
author Marcos César Lima de Mendonça
Ana Maria de Amorim Ferreira
Marta Gonçalves Matos dos Santos
Elva Cristina Oviedo
Maria Sônia Dal Bello
Marilda Mendonça Siqueira
Juan Manuel Piñeiro Maceira
Maria Genoveva von Hubinger
José Nelson dos Santos Silva Couceiro
spellingShingle Marcos César Lima de Mendonça
Ana Maria de Amorim Ferreira
Marta Gonçalves Matos dos Santos
Elva Cristina Oviedo
Maria Sônia Dal Bello
Marilda Mendonça Siqueira
Juan Manuel Piñeiro Maceira
Maria Genoveva von Hubinger
José Nelson dos Santos Silva Couceiro
Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing
Memórias do Instituto Oswaldo Cruz.
parvovirus B19
nucleotide sequencing
genotyping
HMA
SSCP
author_facet Marcos César Lima de Mendonça
Ana Maria de Amorim Ferreira
Marta Gonçalves Matos dos Santos
Elva Cristina Oviedo
Maria Sônia Dal Bello
Marilda Mendonça Siqueira
Juan Manuel Piñeiro Maceira
Maria Genoveva von Hubinger
José Nelson dos Santos Silva Couceiro
author_sort Marcos César Lima de Mendonça
title Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing
title_short Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing
title_full Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing
title_fullStr Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing
title_full_unstemmed Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing
title_sort genotyping of human parvovirus b19 in clinical samples from brazil and paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing
publisher Instituto Oswaldo Cruz, Ministério da Saúde
series Memórias do Instituto Oswaldo Cruz.
issn 1678-8060
publishDate 2011-06-01
description Heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing were utilised to genotype human parvovirus B19 samples from Brazil and Paraguay. Ninety-seven serum samples were collected from individuals presenting with abortion or erythema infectiosum, arthropathies, severe anaemia and transient aplastic crisis; two additional skin samples were collected by biopsy. After the procedure, all clinical samples were classified as genotype 1.
topic parvovirus B19
nucleotide sequencing
genotyping
HMA
SSCP
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400019&lng=en&tlng=en
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