The spermatogenic process of the common vampire bat Desmodus rotundus under a histomorphometric view.

Among all bat species, Desmodus rotundus stands out as one of the most intriguing due to its exclusively haematophagous feeding habits. However, little is known about their spermatogenic cycle. This study aimed at describing the spermatogenic process of common vampire bats through testicular histomo...

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Main Authors: Danielle Barbosa Morais, Luciano Carlos Heringer Porcaro Puga, Tarcízio Antônio Rêgo de Paula, Mariella Bontempo Duca Freitas, Sérgio Luis Pinto da Matta
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5354406?pdf=render
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spelling doaj-094cbdf2a3cc424b80d0cbb23d922a4d2020-11-25T02:23:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01123e017385610.1371/journal.pone.0173856The spermatogenic process of the common vampire bat Desmodus rotundus under a histomorphometric view.Danielle Barbosa MoraisLuciano Carlos Heringer Porcaro PugaTarcízio Antônio Rêgo de PaulaMariella Bontempo Duca FreitasSérgio Luis Pinto da MattaAmong all bat species, Desmodus rotundus stands out as one of the most intriguing due to its exclusively haematophagous feeding habits. However, little is known about their spermatogenic cycle. This study aimed at describing the spermatogenic process of common vampire bats through testicular histomorphometric characterization of adult specimens, spermatogenic production indexes, description of stages of the seminiferous epithelium cycle and estimative of the spermatogenic process duration. Morphometrical and immunohistochemical analyzes for bromodeoxiuridine were conducted under light microscopy and ultrastructural analyzes were performed under transmission electron microscopy. Vampire bats showed higher investment in gonadal tissue (gonadosomatic index of 0.54%) and in seminiferous tubules (tubulesomatic index of 0.49%) when compared to larger mammals. They also showed a high tubular length per gram of testis (34.70 m). Approximately half of the intertubular compartment was found to be comprised by Leydig cells (51.20%), and an average of 23.77x106 of these cells was found per gram of testis. The germline cells showed 16.93% of mitotic index and 2.51% of meiotic index. The overall yield of spermatogenesis was 60% and the testicular spermatic reserve was 71.44x107 spermatozoa per gram of testis. With a total spermatogenesis duration estimated at 37.02 days, vampire bats showed a daily sperm production of 86.80x106 gametes per gram of testis. These findings demonstrate a high sperm production, which is commonly observed in species with promiscuous mating system.http://europepmc.org/articles/PMC5354406?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Danielle Barbosa Morais
Luciano Carlos Heringer Porcaro Puga
Tarcízio Antônio Rêgo de Paula
Mariella Bontempo Duca Freitas
Sérgio Luis Pinto da Matta
spellingShingle Danielle Barbosa Morais
Luciano Carlos Heringer Porcaro Puga
Tarcízio Antônio Rêgo de Paula
Mariella Bontempo Duca Freitas
Sérgio Luis Pinto da Matta
The spermatogenic process of the common vampire bat Desmodus rotundus under a histomorphometric view.
PLoS ONE
author_facet Danielle Barbosa Morais
Luciano Carlos Heringer Porcaro Puga
Tarcízio Antônio Rêgo de Paula
Mariella Bontempo Duca Freitas
Sérgio Luis Pinto da Matta
author_sort Danielle Barbosa Morais
title The spermatogenic process of the common vampire bat Desmodus rotundus under a histomorphometric view.
title_short The spermatogenic process of the common vampire bat Desmodus rotundus under a histomorphometric view.
title_full The spermatogenic process of the common vampire bat Desmodus rotundus under a histomorphometric view.
title_fullStr The spermatogenic process of the common vampire bat Desmodus rotundus under a histomorphometric view.
title_full_unstemmed The spermatogenic process of the common vampire bat Desmodus rotundus under a histomorphometric view.
title_sort spermatogenic process of the common vampire bat desmodus rotundus under a histomorphometric view.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2017-01-01
description Among all bat species, Desmodus rotundus stands out as one of the most intriguing due to its exclusively haematophagous feeding habits. However, little is known about their spermatogenic cycle. This study aimed at describing the spermatogenic process of common vampire bats through testicular histomorphometric characterization of adult specimens, spermatogenic production indexes, description of stages of the seminiferous epithelium cycle and estimative of the spermatogenic process duration. Morphometrical and immunohistochemical analyzes for bromodeoxiuridine were conducted under light microscopy and ultrastructural analyzes were performed under transmission electron microscopy. Vampire bats showed higher investment in gonadal tissue (gonadosomatic index of 0.54%) and in seminiferous tubules (tubulesomatic index of 0.49%) when compared to larger mammals. They also showed a high tubular length per gram of testis (34.70 m). Approximately half of the intertubular compartment was found to be comprised by Leydig cells (51.20%), and an average of 23.77x106 of these cells was found per gram of testis. The germline cells showed 16.93% of mitotic index and 2.51% of meiotic index. The overall yield of spermatogenesis was 60% and the testicular spermatic reserve was 71.44x107 spermatozoa per gram of testis. With a total spermatogenesis duration estimated at 37.02 days, vampire bats showed a daily sperm production of 86.80x106 gametes per gram of testis. These findings demonstrate a high sperm production, which is commonly observed in species with promiscuous mating system.
url http://europepmc.org/articles/PMC5354406?pdf=render
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