MOLECULAR-BIOLOGICAL MARKERS OF HEPATITIS В IN PATIENTS WITH LIVER FIBROSIS/CIRRHOSIS IN UZBEKISTAN

• Main Authors: Yu. V. Ostankova, A. V. Semenov, Kh. N. Faizullaev, E. I. Kazakova, A. V. Kozlov, E. I. Musabaev, A. A. Totolyan
• Format: Article
• Language: Russian
• Published: Central Research Institute for Epidemiology 2016-10-01
• Series: Журнал микробиологии, эпидемиологии и иммунобиологии
• Subjects: hepatitis b; deep genotyping; sequencing; molecular epidemiology; uzbekistan
• Online Access: https://microbiol.elpub.ru/jour/article/view/85
• ISSN: 0372-9311; 2686-7613

Aim. Evaluate prevalence of genetic variants of hepatitis В viruses in population of various regions of Uzbekistan with hepatitis of various genesis and different severity levels of liver fibrosis and cirrhosis. Materials and methods. Blood plasma and liver biopsy from 39 patients with different severity levels of liver fibrosis and cirrhosis served as study material. Genotyping based on direct sequencing of Pre-Sl/Pre-S2/S HBV DNA region was applied. Results. Hepatitis В virus was detected in 32 samples ofthe 39 provided, frequency of occurrence - 82%, respectively. Phylogenetic analysis has shown, that only genotype D was detected among the examined patients, hepatitis В virus subtype D1 predominated (84.38%) compared with D2 (3.12%) and D3 (12.5%) subtypes. Conclusion. Large-scale sequencing of HBV in Central Asia will allow to evaluate routes of transmission and time of evolutionary separation of virus isolates. Understanding the epidemiology of the infectious process is important for development of programs for prophylaxis and therapy of the infection.