Evidence for Chlamydiaceae and Parachlamydiaceae in a wild boar (Sus scrofa) population in Italy

• Main Authors: Antonietta Di Francesco, Raffaella Baldelli, Manuela Donati, Claudia Cotti, Patrizia Bassi, Mauro Delogu
• Format: Article
• Language: English
• Published: Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale 2013-03-01
• Series: Veterinaria Italiana
• Subjects: Chlamydiaceae; Italy; Nested PCR; Parachlamydiaceae; Wild boar
• Online Access: http://www.izs.it/vet_italiana/2013/49_1/119.pdf
• ISSN: 0505-401X; 1828-1427

Conjunctival swabs from 44 free-living wild boars culled during a demographic control programme applied in a Regional Park located in the Northern Italy were examined by 16S rRNA encoding gene nested PCR. In total, 22 (50%) wild boars were PCR positive. Sequencing of the amplicons identified Chlamydia suis and Chlamydia pecorum in 12 and 5 samples, respectively. For one sample found PCR positive, the nucleotide sequence could not be determined. Four conjunctival samples showed ≥ 92% sequence similarities to 16S rRNA sequences from Chlamydia-like organisms, as did large intestine, uterus, and vaginal swabs from the same four animals. Amoeba DNA was found in one Chlamydia-like organism positive conjunctival swab. To our knowledge, this is the first detection of members of the Parachlamydiaceae family in wild boars, confirming a large animal host range for Chlamydia-like organisms.