A semiquantitative analysis technique regarding immunohistochemical detection for matrix metalloproteinases
Modern image processing techniques are used today in order to evaluate immunohistochemical detection for various markers, especially those important for malignant tumor diagnosis and remodeling processes. Evaluating the immunohistochemical markers detection may be completed by a quantitative analysi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
"Alexandru Ioan Cuza" University of Iași
2010-06-01
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| Series: | Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara |
| Online Access: | http://www.gbm.bio.uaic.ro/index.php/gbm/article/view/883 |
| Summary: | Modern image processing techniques are used today in order to evaluate immunohistochemical detection for
various markers, especially those important for malignant tumor diagnosis and remodeling processes. Evaluating the
immunohistochemical markers detection may be completed by a quantitative analysis. We have used samples of normal
and fibrous tissue from surgical scars harvested after 2 months from surgery. We have investigated the
immunohistochemically marked areas and we have performed a semiquantitative image analysis, using an academic,
open sourcesoftware, ImageJ v. 1.38. After image adjustments (binarization) and correction, we have applied a
deconvolution filter after which we have performed and analyzed a histogram of the selected area. The binarized areas
were measured and compared for three samples of each tissue. We have followed the semiquantitative analysis of MMP-2
and MMP-9 presence on the investigated samples. This technique, even if controversial, allows us a fast analysis of
common markers detected by immunohistochemistry |
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| ISSN: | 1582-3571 2248-3276 |