1H-31P home-built solid-state NMR probe with a scroll coil for 400-MHz NB magnet for biological lossy sample

• Main Authors: Ji-Ho Jeong, Minseon Kim, Jinyoung Son, Yongae Kim
• Format: Article
• Language: English
• Published: SpringerOpen 2020-06-01
• Series: Journal of Analytical Science and Technology
• Subjects: Home-built solid-state NMR probe; Biological lossy sample; 1H-31P double resonance; Scroll coil; Membranes
• Online Access: http://link.springer.com/article/10.1186/s40543-020-00224-8

Abstract Phosphorus is one of the most important constituents of human biofilms, in particular lipid bilayer packing, phase transition (gel phase, physiological liquid crystal phase, ripple phase, non-biphasic), lipid head group orientation/mechanics, and pure lipid bilayers. Phosphorus observations in biofilms play an important role in the study of the interaction of many types of proteins and biofilms in the human body. The design and construction of a 1H-31P double resonance probe with a scroll coil for solid-state NMR experiment are introduced. For good efficiency at the relatively high frequency, minimal RF sample heating during the experiment with a lossy sample, and a wide range of tuning frequency, this probe adapted the low inductance of a scroll coil. The 31P NMR spectra obtained on the biological lossy sample were used to demonstrate the possibility for monitoring the protein dynamics on biomembrane mimetic system and phase change of phospholipid.