Structural snapshots of human DNA polymerase μ engaged on a DNA double-strand break

Polymerase μ (Polμ) participates in the repair of DNA double-strand breaks (DSBs) via the nonhomologous end-joining (NHEJ) pathway. Here, the authors determine the crystal structure of a pre-catalytic ternary complex of human Polμ with a bound DSB substrate and they obtain further mechanistic insigh...

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Main Authors:	Andrea M. Kaminski, John M. Pryor, Dale A. Ramsden, Thomas A. Kunkel, Lars C. Pedersen, Katarzyna Bebenek
Format:	Article
Language:	English
Published:	Nature Publishing Group 2020-09-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-020-18506-5

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spelling	doaj-08bcf0ae3cab403fbdaf9bdc81fef3242021-09-26T11:46:48ZengNature Publishing GroupNature Communications2041-17232020-09-0111111010.1038/s41467-020-18506-5Structural snapshots of human DNA polymerase μ engaged on a DNA double-strand breakAndrea M. Kaminski0John M. Pryor1Dale A. Ramsden2Thomas A. Kunkel3Lars C. Pedersen4Katarzyna Bebenek5Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of HealthCurriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, 32-046 Lineberger Comprehensive Cancer CenterCurriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, 32-046 Lineberger Comprehensive Cancer CenterGenome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of HealthGenome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of HealthGenome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of HealthPolymerase μ (Polμ) participates in the repair of DNA double-strand breaks (DSBs) via the nonhomologous end-joining (NHEJ) pathway. Here, the authors determine the crystal structure of a pre-catalytic ternary complex of human Polμ with a bound DSB substrate and they obtain further mechanistic insights by allowing the insertion reaction to proceed in crystallo, which enabled them to determine a Polμ structure with incomplete incorporation and the structure of the post-catalytic nicked state.https://doi.org/10.1038/s41467-020-18506-5
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Andrea M. Kaminski John M. Pryor Dale A. Ramsden Thomas A. Kunkel Lars C. Pedersen Katarzyna Bebenek
spellingShingle	Andrea M. Kaminski John M. Pryor Dale A. Ramsden Thomas A. Kunkel Lars C. Pedersen Katarzyna Bebenek Structural snapshots of human DNA polymerase μ engaged on a DNA double-strand break Nature Communications
author_facet	Andrea M. Kaminski John M. Pryor Dale A. Ramsden Thomas A. Kunkel Lars C. Pedersen Katarzyna Bebenek
author_sort	Andrea M. Kaminski
title	Structural snapshots of human DNA polymerase μ engaged on a DNA double-strand break
title_short	Structural snapshots of human DNA polymerase μ engaged on a DNA double-strand break
title_full	Structural snapshots of human DNA polymerase μ engaged on a DNA double-strand break
title_fullStr	Structural snapshots of human DNA polymerase μ engaged on a DNA double-strand break
title_full_unstemmed	Structural snapshots of human DNA polymerase μ engaged on a DNA double-strand break
title_sort	structural snapshots of human dna polymerase μ engaged on a dna double-strand break
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2020-09-01
description	Polymerase μ (Polμ) participates in the repair of DNA double-strand breaks (DSBs) via the nonhomologous end-joining (NHEJ) pathway. Here, the authors determine the crystal structure of a pre-catalytic ternary complex of human Polμ with a bound DSB substrate and they obtain further mechanistic insights by allowing the insertion reaction to proceed in crystallo, which enabled them to determine a Polμ structure with incomplete incorporation and the structure of the post-catalytic nicked state.
url	https://doi.org/10.1038/s41467-020-18506-5
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Structural snapshots of human DNA polymerase μ engaged on a DNA double-strand break

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