Effects of histamine and sodium hypochlorite on free radical processes in lung tissues of rats

• Main Authors: O. I. Bishko, N. P. Holovchak, D. I. Sanahurs’kyj
• Format: Article
• Language: English
• Published: Львівський національний університет імені Івана Франка 2013-12-01
• Series: Біологічні студії
• Subjects: histamine; sodium hypochlorite; lipid peroxidation; superoxide dismutase; catalase; glutathion peroxidase; lung
• Online Access: http://publications.lnu.edu.ua/journals/index.php/biology/article/view/270

Influence of histamine and sodium hypochlorite on the processes of lipid peroxidation and status of antioxidant defence system of rats lungs was estimated. We found that after exogenous injection of histamine and sodium hypochlorite, content of the endogenous histamine increased during the experiment. It was shown that applied chemicals led to overaccumulation of lipid peroxidation products and disfunction in the antioxidant enzymes. During studying the activities of antioxidant enzymes of lung homogenates, it was found that the activity of superoxide dismutase increased on the 1st day of experiment under influence of histamine (1 µg/kg and 8 µg/kg). However, after 21 days of experiment its activity decreased and reached control values. Under the action of histamine and sodium hypochlorite in concentration of 1 µg/kg, superoxide dismutase activity increased. At the 7th day of experiment, its activity increased. High concentration of histamine and the action of sodium hypochlorite in lung tissues of rats caused a significant activation of superoxide dismutase on the 1st day of experiment, and this index decreased and became lower than control one at the 7th and 14th days. Under the action of histamine and sodium hypochlorite, catalase activity increased compared to control indices. At the same time, glutathione peroxidase activity increased on the 7th day of experiment with its further decrease.