Microbial biofilm root canals and new approaches to the diagnosis and treatment of the chronic forms of pulpitis using a photoactivatable disinfection and ultrasonic treatment

• Format: Article
• Language: Russian
• Published: LLC "Endo Press" 2020-04-01
• Series: Эндодонтия Today
• Subjects: хронический пульпит; корневые каналы; микробная биопленка; эндодонтическое лечение; ультразвуковая ирригация; фотодинамическая терапия; антимикробная активность in vitro; chronic pulpitis; root canals; microbial biofilm; endodontic treatment is ultrasonic irrigation; photodynamic therapy; scanning microscopy; antimicrobial activity in vivo
• Online Access: https://www.endodont.ru/jour/article/view/282

Clinical and laboratory study and evaluation of the effectiveness of ultrasonic treatment of the root canal system in combination with photodynamic therapy, in relation to the microflora of root canals in vitro and in the process of complex treatment of patients with inflammatory diseases of dental pulp. In the control subgroups, we conducted instrumental and medicamental processing of root canals. In the comparison group were given the combined treatment with ultrasonic activation of irrigant and further photodynamic treatment. Evaluate the influence of ultrasonic treatment in combination with photoactivatable system disinfection was performed according to the frequency of the diagnosed complications in the treatment of various forms of chronic pulpitis. The structure of the biofilm root canals was studied using scanning electron microscopy (SEM) using a microscope Quantum 3D (USA). Statistical processing of data in 5 replicates and were processed by parametric statistics for small samples by Manna-Whitney test (p < 0,05). When performing scanning electron microscopy revealed the existence of high level of microbial contamination of areas of the root canal and dentinal tubules. In subgroups, where it was used the standard method of treatment, the percentage of re-treatment and surgical intervention prevails compared to the study group. According to the results of clinical and microbiological studies have observed a positive trend to reduce the number of the designated pathogens and the use of complex interaction of ULTRASOUND and PDT was the most successful method compared to traditional method of treatment