Solid-phase synthesis of protein-polymers on reversible immobilization supports

Solid-phase synthesis of protein-polymers on reversible immobilization supports

Synthesis of protein-polymer conjugates typically relies on multi-step processes in solution and on challenging purification strategies. Here the authors show a robust synthesis approach which eliminates purification processes by immobilizing proteins reversibly on modified agarose beads before graf...

Full description

Bibliographic Details
Main Authors: Hironobu Murata, Sheiliza Carmali, Stefanie L. Baker, Krzysztof Matyjaszewski, Alan J. Russell
Format: Article
Language:English
Published: Nature Publishing Group 2018-02-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-03153-8
id doaj-08770c065c46456a829a399cd1350994
record_format Article
spelling doaj-08770c065c46456a829a399cd13509942021-05-11T10:08:21ZengNature Publishing GroupNature Communications2041-17232018-02-019111010.1038/s41467-018-03153-8Solid-phase synthesis of protein-polymers on reversible immobilization supportsHironobu Murata0Sheiliza Carmali1Stefanie L. Baker2Krzysztof Matyjaszewski3Alan J. Russell4Center for Polymer-Based Protein Engineering, Carnegie Mellon UniversityCenter for Polymer-Based Protein Engineering, Carnegie Mellon UniversityCenter for Polymer-Based Protein Engineering, Carnegie Mellon UniversityCenter for Polymer-Based Protein Engineering, Carnegie Mellon UniversityCenter for Polymer-Based Protein Engineering, Carnegie Mellon UniversitySynthesis of protein-polymer conjugates typically relies on multi-step processes in solution and on challenging purification strategies. Here the authors show a robust synthesis approach which eliminates purification processes by immobilizing proteins reversibly on modified agarose beads before grafting from polymers via ATRP.https://doi.org/10.1038/s41467-018-03153-8
collection DOAJ
language English
format Article
sources DOAJ
author Hironobu Murata
Sheiliza Carmali
Stefanie L. Baker
Krzysztof Matyjaszewski
Alan J. Russell
spellingShingle Hironobu Murata
Sheiliza Carmali
Stefanie L. Baker
Krzysztof Matyjaszewski
Alan J. Russell
Solid-phase synthesis of protein-polymers on reversible immobilization supports
Nature Communications
author_facet Hironobu Murata
Sheiliza Carmali
Stefanie L. Baker
Krzysztof Matyjaszewski
Alan J. Russell
author_sort Hironobu Murata
title Solid-phase synthesis of protein-polymers on reversible immobilization supports
title_short Solid-phase synthesis of protein-polymers on reversible immobilization supports
title_full Solid-phase synthesis of protein-polymers on reversible immobilization supports
title_fullStr Solid-phase synthesis of protein-polymers on reversible immobilization supports
title_full_unstemmed Solid-phase synthesis of protein-polymers on reversible immobilization supports
title_sort solid-phase synthesis of protein-polymers on reversible immobilization supports
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2018-02-01
description Synthesis of protein-polymer conjugates typically relies on multi-step processes in solution and on challenging purification strategies. Here the authors show a robust synthesis approach which eliminates purification processes by immobilizing proteins reversibly on modified agarose beads before grafting from polymers via ATRP.
url https://doi.org/10.1038/s41467-018-03153-8
work_keys_str_mv AT hironobumurata solidphasesynthesisofproteinpolymersonreversibleimmobilizationsupports
AT sheilizacarmali solidphasesynthesisofproteinpolymersonreversibleimmobilizationsupports
AT stefanielbaker solidphasesynthesisofproteinpolymersonreversibleimmobilizationsupports
AT krzysztofmatyjaszewski solidphasesynthesisofproteinpolymersonreversibleimmobilizationsupports
AT alanjrussell solidphasesynthesisofproteinpolymersonreversibleimmobilizationsupports
_version_ 1721448574712020992

Similar Items