The Marine <i>Catenovulum agarivorans</i> MNH15 and Dextranase: Removing Dental Plaque

The Marine <i>Catenovulum agarivorans</i> MNH15 and Dextranase: Removing Dental Plaque

Dextranase, a hydrolase that specifically hydrolyzes &#945;-1,6-glucosidic bonds, has been used in the pharmaceutical, food, and biotechnology industries. In this study, the strain of <i>Catenovulum agarivorans</i> MNH15 was screened from marine samples. When the temperature, initial...

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Main Authors: Xiaohua Lai, Xin Liu, Xueqin Liu, Tian Deng, Yanli Feng, Xiaopeng Tian, Mingsheng Lyu, Shujun Wang
Format: Article
Language:English
Published: MDPI AG 2019-10-01
Series:Marine Drugs
Subjects:
<i>catenovulum agarivorans</i>
dextranase
dental plaque
oligo-saccharides
Online Access:https://www.mdpi.com/1660-3397/17/10/592
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spelling doaj-0854410a53e04f09b08369beee9ae0dd2020-11-24T22:10:24ZengMDPI AGMarine Drugs1660-33972019-10-01171059210.3390/md17100592md17100592The Marine <i>Catenovulum agarivorans</i> MNH15 and Dextranase: Removing Dental PlaqueXiaohua Lai0Xin Liu1Xueqin Liu2Tian Deng3Yanli Feng4Xiaopeng Tian5Mingsheng Lyu6Shujun Wang7Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaDextranase, a hydrolase that specifically hydrolyzes &#945;-1,6-glucosidic bonds, has been used in the pharmaceutical, food, and biotechnology industries. In this study, the strain of <i>Catenovulum agarivorans</i> MNH15 was screened from marine samples. When the temperature, initial pH, NaCl concentration, and inducer concentration were 30 &#176;C, 8.0, 5 g/L, and 8 g/L, respectively, it yielded more dextranase. The molecular weight of the dextranase was approximately 110 kDa. The maximum enzyme activity was achieved at 40 &#176;C and a pH of 8.0. The enzyme was stable at 30 &#176;C and a pH of 5&#8722;9. The metal ion Sr<sup>2+</sup> enhanced its activity, whereas NH<sup>4+</sup>, Co<sup>2+</sup>, Cu<sup>2+</sup>, and Li<sup>+</sup> had the opposite effect. The dextranase effectively inhibited the formation of biofilm by <i>Streptococcus mutans</i>. Moreover, sodium fluoride, xylitol, and sodium benzoate, all used in dental care products, had no significant effect on dextranase activity. In addition, high-performance liquid chromatography (HPLC) showed that dextran was mainly hydrolyzed to glucose, maltose, and maltoheptaose. The results indicated that dextranase has high application potential in dental products such as toothpaste and mouthwash.https://www.mdpi.com/1660-3397/17/10/592<i>catenovulum agarivorans</i>dextranasedental plaqueoligo-saccharides
collection DOAJ
language English
format Article
sources DOAJ
author Xiaohua Lai
Xin Liu
Xueqin Liu
Tian Deng
Yanli Feng
Xiaopeng Tian
Mingsheng Lyu
Shujun Wang
spellingShingle Xiaohua Lai
Xin Liu
Xueqin Liu
Tian Deng
Yanli Feng
Xiaopeng Tian
Mingsheng Lyu
Shujun Wang
The Marine <i>Catenovulum agarivorans</i> MNH15 and Dextranase: Removing Dental Plaque
Marine Drugs
<i>catenovulum agarivorans</i>
dextranase
dental plaque
oligo-saccharides
author_facet Xiaohua Lai
Xin Liu
Xueqin Liu
Tian Deng
Yanli Feng
Xiaopeng Tian
Mingsheng Lyu
Shujun Wang
author_sort Xiaohua Lai
title The Marine <i>Catenovulum agarivorans</i> MNH15 and Dextranase: Removing Dental Plaque
title_short The Marine <i>Catenovulum agarivorans</i> MNH15 and Dextranase: Removing Dental Plaque
title_full The Marine <i>Catenovulum agarivorans</i> MNH15 and Dextranase: Removing Dental Plaque
title_fullStr The Marine <i>Catenovulum agarivorans</i> MNH15 and Dextranase: Removing Dental Plaque
title_full_unstemmed The Marine <i>Catenovulum agarivorans</i> MNH15 and Dextranase: Removing Dental Plaque
title_sort marine <i>catenovulum agarivorans</i> mnh15 and dextranase: removing dental plaque
publisher MDPI AG
series Marine Drugs
issn 1660-3397
publishDate 2019-10-01
description Dextranase, a hydrolase that specifically hydrolyzes &#945;-1,6-glucosidic bonds, has been used in the pharmaceutical, food, and biotechnology industries. In this study, the strain of <i>Catenovulum agarivorans</i> MNH15 was screened from marine samples. When the temperature, initial pH, NaCl concentration, and inducer concentration were 30 &#176;C, 8.0, 5 g/L, and 8 g/L, respectively, it yielded more dextranase. The molecular weight of the dextranase was approximately 110 kDa. The maximum enzyme activity was achieved at 40 &#176;C and a pH of 8.0. The enzyme was stable at 30 &#176;C and a pH of 5&#8722;9. The metal ion Sr<sup>2+</sup> enhanced its activity, whereas NH<sup>4+</sup>, Co<sup>2+</sup>, Cu<sup>2+</sup>, and Li<sup>+</sup> had the opposite effect. The dextranase effectively inhibited the formation of biofilm by <i>Streptococcus mutans</i>. Moreover, sodium fluoride, xylitol, and sodium benzoate, all used in dental care products, had no significant effect on dextranase activity. In addition, high-performance liquid chromatography (HPLC) showed that dextran was mainly hydrolyzed to glucose, maltose, and maltoheptaose. The results indicated that dextranase has high application potential in dental products such as toothpaste and mouthwash.
topic <i>catenovulum agarivorans</i>
dextranase
dental plaque
oligo-saccharides
url https://www.mdpi.com/1660-3397/17/10/592
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