In Vivo Quantitative Vasculature Segmentation and Assessment for Photodynamic Therapy Process Monitoring Using Photoacoustic Microscopy

In Vivo Quantitative Vasculature Segmentation and Assessment for Photodynamic Therapy Process Monitoring Using Photoacoustic Microscopy

Vascular damage is one of the therapeutic mechanisms of photodynamic therapy (PDT). In particular, short-term PDT treatments can effectively destroy malignant lesions while minimizing damage to nonmalignant tissue. In this study, we investigate the feasibility of label-free quantitative photoacousti...

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Main Authors: Thi Thao Mai, Su Woong Yoo, Suhyun Park, Jin Young Kim, Kang-Ho Choi, Chulhong Kim, Seong Young Kwon, Jung-Joon Min, Changho Lee
Format: Article
Language:English
Published: MDPI AG 2021-03-01
Series:Sensors
Subjects:
photodynamic therapy
photoacoustic microscopy
quantitative analysis
Online Access:https://www.mdpi.com/1424-8220/21/5/1776
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spelling doaj-08280ab74abb457392cb5224a6171ba72021-03-05T00:04:03ZengMDPI AGSensors1424-82202021-03-01211776177610.3390/s21051776In Vivo Quantitative Vasculature Segmentation and Assessment for Photodynamic Therapy Process Monitoring Using Photoacoustic MicroscopyThi Thao Mai0Su Woong Yoo1Suhyun Park2Jin Young Kim3Kang-Ho Choi4Chulhong Kim5Seong Young Kwon6Jung-Joon Min7Changho Lee8Department of Artificial Intelligence Convergence, Chonnam National University, Gwangju 61186, KoreaDepartment of Nuclear Medicine, Chonnam National University Hwasun Hospital, Hwasun, Jeollanamdo 58128, KoreaInterdisciplinary Program of Molecular Medicine, Chonnam National University, Gwangju 61186, KoreaDepartment of Creative IT Engineering and Electrical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk-do 37673, KoreaDepartment of Neurology, Chonnam National University Hospital, 8 Hak-dong, Dong-gu, Gwangju 501-757, KoreaDepartment of Creative IT Engineering and Electrical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk-do 37673, KoreaDepartment of Nuclear Medicine, Chonnam National University Hwasun Hospital, Hwasun, Jeollanamdo 58128, KoreaDepartment of Nuclear Medicine, Chonnam National University Hwasun Hospital, Hwasun, Jeollanamdo 58128, KoreaDepartment of Artificial Intelligence Convergence, Chonnam National University, Gwangju 61186, KoreaVascular damage is one of the therapeutic mechanisms of photodynamic therapy (PDT). In particular, short-term PDT treatments can effectively destroy malignant lesions while minimizing damage to nonmalignant tissue. In this study, we investigate the feasibility of label-free quantitative photoacoustic microscopy (PAM) for monitoring the vasculature changes under the effect of PDT in mouse ear melanoma tumors. In particular, quantitative vasculature evaluation was conducted based on Hessian filter segmentation. Three-dimensional morphological PAM and depth-resolved images before and after PDT treatment were acquired. In addition, five quantitative vasculature parameters, including the PA signal, vessel diameter, vessel density, perfused vessel density, and vessel complexity, were analyzed to evaluate the influence of PDT on four different areas: Two melanoma tumors, and control and normal vessel areas. The quantitative and qualitative results successfully demonstrated the potential of the proposed PAM-based quantitative approach to evaluate the effectiveness of the PDT method.https://www.mdpi.com/1424-8220/21/5/1776photodynamic therapyphotoacoustic microscopyquantitative analysis
collection DOAJ
language English
format Article
sources DOAJ
author Thi Thao Mai
Su Woong Yoo
Suhyun Park
Jin Young Kim
Kang-Ho Choi
Chulhong Kim
Seong Young Kwon
Jung-Joon Min
Changho Lee
spellingShingle Thi Thao Mai
Su Woong Yoo
Suhyun Park
Jin Young Kim
Kang-Ho Choi
Chulhong Kim
Seong Young Kwon
Jung-Joon Min
Changho Lee
In Vivo Quantitative Vasculature Segmentation and Assessment for Photodynamic Therapy Process Monitoring Using Photoacoustic Microscopy
Sensors
photodynamic therapy
photoacoustic microscopy
quantitative analysis
author_facet Thi Thao Mai
Su Woong Yoo
Suhyun Park
Jin Young Kim
Kang-Ho Choi
Chulhong Kim
Seong Young Kwon
Jung-Joon Min
Changho Lee
author_sort Thi Thao Mai
title In Vivo Quantitative Vasculature Segmentation and Assessment for Photodynamic Therapy Process Monitoring Using Photoacoustic Microscopy
title_short In Vivo Quantitative Vasculature Segmentation and Assessment for Photodynamic Therapy Process Monitoring Using Photoacoustic Microscopy
title_full In Vivo Quantitative Vasculature Segmentation and Assessment for Photodynamic Therapy Process Monitoring Using Photoacoustic Microscopy
title_fullStr In Vivo Quantitative Vasculature Segmentation and Assessment for Photodynamic Therapy Process Monitoring Using Photoacoustic Microscopy
title_full_unstemmed In Vivo Quantitative Vasculature Segmentation and Assessment for Photodynamic Therapy Process Monitoring Using Photoacoustic Microscopy
title_sort in vivo quantitative vasculature segmentation and assessment for photodynamic therapy process monitoring using photoacoustic microscopy
publisher MDPI AG
series Sensors
issn 1424-8220
publishDate 2021-03-01
description Vascular damage is one of the therapeutic mechanisms of photodynamic therapy (PDT). In particular, short-term PDT treatments can effectively destroy malignant lesions while minimizing damage to nonmalignant tissue. In this study, we investigate the feasibility of label-free quantitative photoacoustic microscopy (PAM) for monitoring the vasculature changes under the effect of PDT in mouse ear melanoma tumors. In particular, quantitative vasculature evaluation was conducted based on Hessian filter segmentation. Three-dimensional morphological PAM and depth-resolved images before and after PDT treatment were acquired. In addition, five quantitative vasculature parameters, including the PA signal, vessel diameter, vessel density, perfused vessel density, and vessel complexity, were analyzed to evaluate the influence of PDT on four different areas: Two melanoma tumors, and control and normal vessel areas. The quantitative and qualitative results successfully demonstrated the potential of the proposed PAM-based quantitative approach to evaluate the effectiveness of the PDT method.
topic photodynamic therapy
photoacoustic microscopy
quantitative analysis
url https://www.mdpi.com/1424-8220/21/5/1776
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