Effects of Modifiers on Physiological Metabolism of <i>Lolium perenne</i> Seedlings in Diesel-Polluted Soils

• Main Authors: ZHAO Xuan, HE Xing-dong, ZHANG Jing-lei
• Format: Article
• Language: zho
• Published: Agro-Environmental Protection Institute, Ministry of Agriculture 2017-06-01
• Series: Journal of Agricultural Resources and Environment
• Subjects: <i>Lolium perenne</i>, diesel-polluted soil, antioxidant enzymes, chlorophyll, phytoremediation
• Online Access: http://www.aed.org.cn/nyzyyhjxb/html/2017/4/20170411.htm

The pot experiment for single-factor with diesel oil polluted soil and the pot experiment for three-factor orthogonal with sawdust-ammonium nitrate-monopotassium phosphate under diesel oil polluted soil with salt stress, were performed to analyze the activity of antioxidant enzymes and chlorophyll content in <i>Lolium perenne</i> seedlings, and to explore the physiological response of <i>L. perenne</i> seedlings under diesel oil polluted soil and its regulations. The results showed that, soil diesel pollution significantly decreased the biomass. Compared with control, activity of superoxide dismutases(SOD) in leaf decreased significantly at 0.3% and 0.9% soil diesel pollution, peroxidases (POD) and catalase(CAT) in leaf decreased significantly at 0.6% and 0.9% soil diesel pollution, the root SOD activity increased significantly at 0.9% diesel concentration while the root POD activity decreased significantly at 0.6% and 0.9% soil diesel pollution. As for the salinity soil polluted by diesel oil, the activity of POD and CAT in leaf increased significantly at 10% volume fraction of sawdust, and the content of chlorophyll a and chlorophyll b increased significantly as well. Meanwhile, chlorophyll a and chlorophyll b content increased significantly at 0.3 g&#183;kg^-1 amount of ammonium nitrate. Thereby, sawdust and ammonium nitrate addition could effectively improve physiological metabolic of <i>L. perenne</i> seedlings.