α-linolenic acid reduces TNF-induced apoptosis in C2C12 myoblasts by regulating expression of apoptotic proteins

Impaired regeneration and consequent muscle wasting is a major feature of muscle degenerative diseases. Nutritional interventions as adjuvant strategy for preventing such conditions are recently gaining increasing attention. Ingestion of n3-polyunsaturated fatty acids has been suggested to have a po...

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Main Authors: Felicia Carotenuto, Dario Coletti, Paolo Di Nardo, Laura Teodori
Format: Article
Language:English
Published: PAGEPress Publications 2016-11-01
Series:European Journal of Translational Myology
Subjects:
Online Access:http://www.pagepressjournals.org/index.php/bam/article/view/6033
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spelling doaj-0813f66ea1284b70974c353bc33388002020-11-25T02:34:24ZengPAGEPress PublicationsEuropean Journal of Translational Myology2037-74522037-74602016-11-0126410.4081/ejtm.2016.60334997α-linolenic acid reduces TNF-induced apoptosis in C2C12 myoblasts by regulating expression of apoptotic proteinsFelicia Carotenuto0Dario Coletti1Paolo Di Nardo2Laura Teodori3Department Clinical Sciences and Translational Medicine, University of Rome Tor Vergata, Rome; Diagnostics and Metrology Laboratory, FSN-TECFIS-DIM, ENEA-Frascati, RomeDepartment of Biological Adaptation and Aging B2A, University Pierre et Marie Curie Paris 06, Paris, France; Section of Histology and Medical Embryology, Department of Anatomical, Histological, Forensic and Orthopaedic Sciences, Sapienza University of RomeDepartment Clinical Sciences and Translational Medicine, University of Rome Tor Vergata, RomeDiagnostics and Metrology Laboratory, FSN-TECFIS-DIM, ENEA-Frascati, RomeImpaired regeneration and consequent muscle wasting is a major feature of muscle degenerative diseases. Nutritional interventions as adjuvant strategy for preventing such conditions are recently gaining increasing attention. Ingestion of n3-polyunsaturated fatty acids has been suggested to have a positive impact on muscle diseases. We recently demonstrated that the dietary n3-fatty acid, alpha-linolenic acid (ALA), exerts potent beneficial effects in preserving skeletal muscle regeneration in models of muscle dystrophy. To better elucidate the underlying mechanism we investigate here on the expression level of the anti- and pro-apototic proteins, as well as caspase-3 activity, in C2C12 myoblasts challenged with pathological levels of TNF. The results demonstrated that ALA protective effect on C2C12 myoblasts was associated to an increased Bcl-2/Bax ratio. Indeed, the effect of ALA was directed to rescue Bcl-2 expression and decrease Bax expression both affected in an opposite way by TNF treatment. This effect was associated with a decrease in caspase-3 activity by ALA. TNF is a major pro-inflammatory cytokine that is expressed in damaged skeletal muscle, therefore, counteract inflammatory signals in the muscle microenvironment represents a critical strategy to ameliorate skeletal muscle pathologieshttp://www.pagepressjournals.org/index.php/bam/article/view/6033MyoblastsApoptotic proteinsMuscle wastingInflammationPhytochemicals
collection DOAJ
language English
format Article
sources DOAJ
author Felicia Carotenuto
Dario Coletti
Paolo Di Nardo
Laura Teodori
spellingShingle Felicia Carotenuto
Dario Coletti
Paolo Di Nardo
Laura Teodori
α-linolenic acid reduces TNF-induced apoptosis in C2C12 myoblasts by regulating expression of apoptotic proteins
European Journal of Translational Myology
Myoblasts
Apoptotic proteins
Muscle wasting
Inflammation
Phytochemicals
author_facet Felicia Carotenuto
Dario Coletti
Paolo Di Nardo
Laura Teodori
author_sort Felicia Carotenuto
title α-linolenic acid reduces TNF-induced apoptosis in C2C12 myoblasts by regulating expression of apoptotic proteins
title_short α-linolenic acid reduces TNF-induced apoptosis in C2C12 myoblasts by regulating expression of apoptotic proteins
title_full α-linolenic acid reduces TNF-induced apoptosis in C2C12 myoblasts by regulating expression of apoptotic proteins
title_fullStr α-linolenic acid reduces TNF-induced apoptosis in C2C12 myoblasts by regulating expression of apoptotic proteins
title_full_unstemmed α-linolenic acid reduces TNF-induced apoptosis in C2C12 myoblasts by regulating expression of apoptotic proteins
title_sort α-linolenic acid reduces tnf-induced apoptosis in c2c12 myoblasts by regulating expression of apoptotic proteins
publisher PAGEPress Publications
series European Journal of Translational Myology
issn 2037-7452
2037-7460
publishDate 2016-11-01
description Impaired regeneration and consequent muscle wasting is a major feature of muscle degenerative diseases. Nutritional interventions as adjuvant strategy for preventing such conditions are recently gaining increasing attention. Ingestion of n3-polyunsaturated fatty acids has been suggested to have a positive impact on muscle diseases. We recently demonstrated that the dietary n3-fatty acid, alpha-linolenic acid (ALA), exerts potent beneficial effects in preserving skeletal muscle regeneration in models of muscle dystrophy. To better elucidate the underlying mechanism we investigate here on the expression level of the anti- and pro-apototic proteins, as well as caspase-3 activity, in C2C12 myoblasts challenged with pathological levels of TNF. The results demonstrated that ALA protective effect on C2C12 myoblasts was associated to an increased Bcl-2/Bax ratio. Indeed, the effect of ALA was directed to rescue Bcl-2 expression and decrease Bax expression both affected in an opposite way by TNF treatment. This effect was associated with a decrease in caspase-3 activity by ALA. TNF is a major pro-inflammatory cytokine that is expressed in damaged skeletal muscle, therefore, counteract inflammatory signals in the muscle microenvironment represents a critical strategy to ameliorate skeletal muscle pathologies
topic Myoblasts
Apoptotic proteins
Muscle wasting
Inflammation
Phytochemicals
url http://www.pagepressjournals.org/index.php/bam/article/view/6033
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AT dariocoletti alinolenicacidreducestnfinducedapoptosisinc2c12myoblastsbyregulatingexpressionofapoptoticproteins
AT paolodinardo alinolenicacidreducestnfinducedapoptosisinc2c12myoblastsbyregulatingexpressionofapoptoticproteins
AT laurateodori alinolenicacidreducestnfinducedapoptosisinc2c12myoblastsbyregulatingexpressionofapoptoticproteins
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