A cost-effective melting temperature assay for the detection of single-nucleotide polymorphism in the MBL2 gene of HIV-1-infected children

A cost-effective melting temperature assay for the detection of single-nucleotide polymorphism in the MBL2 gene of HIV-1-infected children

We report a fast (less than 3 h) and cost-effective melting temperature assay method for the detection of single-nucleotide polymorphisms in the MBL2 gene. The protocol, which is based on the Corbett Rotor Gene real time PCR platform and SYBR Green I chemistry, yielded, in the cohorts studied, sensi...

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Bibliographic Details
Main Authors:	Arraes L.C., de Souza P.R., Bruneska D., Castelo Filho A., de Souza Cavada B., de Lima Filho J.L., Crovella S.
Format:	Article
Language:	English
Published:	Associação Brasileira de Divulgação Científica 2006-01-01
Series:	Brazilian Journal of Medical and Biological Research
Subjects:	Melting temperature assay Real time PCR MBL2 Polymorphisms HIV-1-positive children
Online Access:	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600003

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