Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing

Delivery of Cas9 ribonucleoprotein complexes can be done via microinjection into eggs, though this is a technically challenging procedure. Here the authors demonstrate ReMOT Control, delivery of RNPs to the mosquito germline by tagging with peptide derived from yolk protein 1.

Bibliographic Details
Main Authors:	Duverney Chaverra-Rodriguez, Vanessa M. Macias, Grant L. Hughes, Sujit Pujhari, Yasutsugu Suzuki, David R. Peterson, Donghun Kim, Sage McKeand, Jason L. Rasgon
Format:	Article
Language:	English
Published:	Nature Publishing Group 2018-08-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-018-05425-9

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spelling	doaj-07ec832a764747dfbd6c8d3b650219682021-05-11T09:27:32ZengNature Publishing GroupNature Communications2041-17232018-08-019111110.1038/s41467-018-05425-9Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editingDuverney Chaverra-Rodriguez0Vanessa M. Macias1Grant L. Hughes2Sujit Pujhari3Yasutsugu Suzuki4David R. Peterson5Donghun Kim6Sage McKeand7Jason L. Rasgon8Department of Entomology, The Huck Institutes of the Life Sciences, and the Center for infectious Disease Dynamics, The Pennsylvania State UniversityDepartment of Entomology, The Huck Institutes of the Life Sciences, and the Center for infectious Disease Dynamics, The Pennsylvania State UniversityDepartment of Entomology, The Huck Institutes of the Life Sciences, and the Center for infectious Disease Dynamics, The Pennsylvania State UniversityDepartment of Entomology, The Huck Institutes of the Life Sciences, and the Center for infectious Disease Dynamics, The Pennsylvania State UniversityDepartment of Entomology, The Huck Institutes of the Life Sciences, and the Center for infectious Disease Dynamics, The Pennsylvania State UniversityDepartment of Entomology, The Huck Institutes of the Life Sciences, and the Center for infectious Disease Dynamics, The Pennsylvania State UniversityDepartment of Entomology, The Huck Institutes of the Life Sciences, and the Center for infectious Disease Dynamics, The Pennsylvania State UniversityDepartment of Entomology, The Huck Institutes of the Life Sciences, and the Center for infectious Disease Dynamics, The Pennsylvania State UniversityDepartment of Entomology, The Huck Institutes of the Life Sciences, and the Center for infectious Disease Dynamics, The Pennsylvania State UniversityDelivery of Cas9 ribonucleoprotein complexes can be done via microinjection into eggs, though this is a technically challenging procedure. Here the authors demonstrate ReMOT Control, delivery of RNPs to the mosquito germline by tagging with peptide derived from yolk protein 1.https://doi.org/10.1038/s41467-018-05425-9
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Duverney Chaverra-Rodriguez Vanessa M. Macias Grant L. Hughes Sujit Pujhari Yasutsugu Suzuki David R. Peterson Donghun Kim Sage McKeand Jason L. Rasgon
spellingShingle	Duverney Chaverra-Rodriguez Vanessa M. Macias Grant L. Hughes Sujit Pujhari Yasutsugu Suzuki David R. Peterson Donghun Kim Sage McKeand Jason L. Rasgon Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing Nature Communications
author_facet	Duverney Chaverra-Rodriguez Vanessa M. Macias Grant L. Hughes Sujit Pujhari Yasutsugu Suzuki David R. Peterson Donghun Kim Sage McKeand Jason L. Rasgon
author_sort	Duverney Chaverra-Rodriguez
title	Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing
title_short	Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing
title_full	Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing
title_fullStr	Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing
title_full_unstemmed	Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing
title_sort	targeted delivery of crispr-cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2018-08-01
description	Delivery of Cas9 ribonucleoprotein complexes can be done via microinjection into eggs, though this is a technically challenging procedure. Here the authors demonstrate ReMOT Control, delivery of RNPs to the mosquito germline by tagging with peptide derived from yolk protein 1.
url	https://doi.org/10.1038/s41467-018-05425-9
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Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing

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