The upstream 5′ splice site remains associated to the transcription machinery during intron synthesis

We know that most splicing reactions take place co-transcriptionally, but how the transcription machinery facilitate splicing of introns is unknown. Here the authors show that the 5′ splice site remains associated with the transcription machinery during intron synthesis through U1 snRNP, providing a...

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Main Authors:	Yodfat Leader, Galit Lev Maor, Matan Sorek, Ronna Shayevitch, Maram Hussein, Ofir Hameiri, Luna Tammer, Jonathan Zonszain, Ifat Keydar, Dror Hollander, Eran Meshorer, Gil Ast
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-07-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-021-24774-6

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spelling	doaj-07b1b9cd06be45f09e950940712792562021-08-01T11:38:49ZengNature Publishing GroupNature Communications2041-17232021-07-0112111110.1038/s41467-021-24774-6The upstream 5′ splice site remains associated to the transcription machinery during intron synthesisYodfat Leader0Galit Lev Maor1Matan Sorek2Ronna Shayevitch3Maram Hussein4Ofir Hameiri5Luna Tammer6Jonathan Zonszain7Ifat Keydar8Dror Hollander9Eran Meshorer10Gil Ast11Department of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel-Aviv UniversityDepartment of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel-Aviv UniversityDepartment of Genetics, The Institute of Life Sciences, and The Edmond and Lily Center for Brain Sciences (ELSC), The Hebrew University of Jerusalem, Edmond J. Safra CampusDepartment of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel-Aviv UniversityDepartment of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel-Aviv UniversityDepartment of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel-Aviv UniversityDepartment of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel-Aviv UniversityDepartment of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel-Aviv UniversityDepartment of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel-Aviv UniversityDepartment of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel-Aviv UniversityDepartment of Genetics, The Institute of Life Sciences, and The Edmond and Lily Center for Brain Sciences (ELSC), The Hebrew University of Jerusalem, Edmond J. Safra CampusDepartment of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel-Aviv UniversityWe know that most splicing reactions take place co-transcriptionally, but how the transcription machinery facilitate splicing of introns is unknown. Here the authors show that the 5′ splice site remains associated with the transcription machinery during intron synthesis through U1 snRNP, providing a basis for the rapid splicing reaction of introns.https://doi.org/10.1038/s41467-021-24774-6
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Yodfat Leader Galit Lev Maor Matan Sorek Ronna Shayevitch Maram Hussein Ofir Hameiri Luna Tammer Jonathan Zonszain Ifat Keydar Dror Hollander Eran Meshorer Gil Ast
spellingShingle	Yodfat Leader Galit Lev Maor Matan Sorek Ronna Shayevitch Maram Hussein Ofir Hameiri Luna Tammer Jonathan Zonszain Ifat Keydar Dror Hollander Eran Meshorer Gil Ast The upstream 5′ splice site remains associated to the transcription machinery during intron synthesis Nature Communications
author_facet	Yodfat Leader Galit Lev Maor Matan Sorek Ronna Shayevitch Maram Hussein Ofir Hameiri Luna Tammer Jonathan Zonszain Ifat Keydar Dror Hollander Eran Meshorer Gil Ast
author_sort	Yodfat Leader
title	The upstream 5′ splice site remains associated to the transcription machinery during intron synthesis
title_short	The upstream 5′ splice site remains associated to the transcription machinery during intron synthesis
title_full	The upstream 5′ splice site remains associated to the transcription machinery during intron synthesis
title_fullStr	The upstream 5′ splice site remains associated to the transcription machinery during intron synthesis
title_full_unstemmed	The upstream 5′ splice site remains associated to the transcription machinery during intron synthesis
title_sort	upstream 5′ splice site remains associated to the transcription machinery during intron synthesis
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2021-07-01
description	We know that most splicing reactions take place co-transcriptionally, but how the transcription machinery facilitate splicing of introns is unknown. Here the authors show that the 5′ splice site remains associated with the transcription machinery during intron synthesis through U1 snRNP, providing a basis for the rapid splicing reaction of introns.
url	https://doi.org/10.1038/s41467-021-24774-6
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The upstream 5′ splice site remains associated to the transcription machinery during intron synthesis

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