Improvement of Thermal Stability via Outer-Loop Ion Pair Interaction of Mutated T1 Lipase from Geobacillus zalihae Strain T1

Improvement of Thermal Stability via Outer-Loop Ion Pair Interaction of Mutated T1 Lipase from Geobacillus zalihae Strain T1

Mutant D311E and K344R were constructed using site-directed mutagenesis to introduce an additional ion pair at the inter-loop and the intra-loop, respectively, to determine the effect of ion pairs on the stability of T1 lipase isolated from Geobacillus zalihae. A series of purification steps was app...

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Bibliographic Details
Main Authors: Mahiran Basri, Rudzanna Ruslan, Raja Noor Zaliha Raja Abd. Rahman, Mohd Shukuri Mohamad Ali, Thean Chor Leow, Abu Bakar Salleh
Format: Article
Language:English
Published: MDPI AG 2012-01-01
Series:International Journal of Molecular Sciences
Subjects:
thermostable lipase
ion pair interaction
thermal stability
CD spectral analysis
lipase crystal
X-ray diffraction
Online Access:http://www.mdpi.com/1422-0067/13/1/943/

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