Proteolytic parameter changes in the plasma of patients with bladder cancer – depending on tumor stage

Bladder cancer (BC) is a worldwide common disease with a high mortality rate. Recognizing the dynamic changes in plasma that proteases and their inhibitors undergo might be valuable in understanding the carcinogenesis of invasive bladder cancer and in identifying BC patients with poor prognosis. Thi...

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Main Authors:	Dmytryk Viktor, Luhovska Tetiana, Yakovlev Pavel, Savchuk Olexiy, Halenova Tetiana, Raksha Nataliia, Ostapchenko Ludmila
Format:	Article
Language:	English
Published:	Sciendo 2020-06-01
Series:	Current Issues in Pharmacy and Medical Sciences
Subjects:	bladder cancer serine proteases mmps α1a α2m medium mass molecules
Online Access:	https://doi.org/10.2478/cipms-2020-0013

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spelling	doaj-07aab31f29534765a4edf602e9e63ac92021-09-06T19:22:04ZengSciendoCurrent Issues in Pharmacy and Medical Sciences2084-980X2300-66762020-06-01332677110.2478/cipms-2020-0013cipms-2020-0013Proteolytic parameter changes in the plasma of patients with bladder cancer – depending on tumor stageDmytryk Viktor0Luhovska Tetiana1Yakovlev Pavel2Savchuk Olexiy3Halenova Tetiana4Raksha Nataliia5Ostapchenko Ludmila6ESC “The Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, UkraineESC “The Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, UkraineDepartment of Urology, O. Bogomolets National Medical University, Kyiv, UkraineESC “The Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, UkraineESC “The Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, UkraineESC “The Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, UkraineESC “The Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, UkraineBladder cancer (BC) is a worldwide common disease with a high mortality rate. Recognizing the dynamic changes in plasma that proteases and their inhibitors undergo might be valuable in understanding the carcinogenesis of invasive bladder cancer and in identifying BC patients with poor prognosis. This study aims to determine the activity of the proteolytic enzyme system and their inhibitors in patients with BC. In this paper, the total proteolytic activity, the activity of matrix metalloproteases (MMPs) and serine proteases was analyzed by the method of caseinolytic activity. For detection of activity of some inhibitors of proteolysis, we used the unified method for determining the activity of alpha-1-antitrypsin (α1A) and alpha-2-Macroglobulin (α2M) in human plasma. The level of medium-mass molecules (MMM) was assessed spectrophotometrically by applying the Nikolaichik method.https://doi.org/10.2478/cipms-2020-0013bladder cancerserine proteasesmmpsα1aα2mmedium mass molecules
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Dmytryk Viktor Luhovska Tetiana Yakovlev Pavel Savchuk Olexiy Halenova Tetiana Raksha Nataliia Ostapchenko Ludmila
spellingShingle	Dmytryk Viktor Luhovska Tetiana Yakovlev Pavel Savchuk Olexiy Halenova Tetiana Raksha Nataliia Ostapchenko Ludmila Proteolytic parameter changes in the plasma of patients with bladder cancer – depending on tumor stage Current Issues in Pharmacy and Medical Sciences bladder cancer serine proteases mmps α1a α2m medium mass molecules
author_facet	Dmytryk Viktor Luhovska Tetiana Yakovlev Pavel Savchuk Olexiy Halenova Tetiana Raksha Nataliia Ostapchenko Ludmila
author_sort	Dmytryk Viktor
title	Proteolytic parameter changes in the plasma of patients with bladder cancer – depending on tumor stage
title_short	Proteolytic parameter changes in the plasma of patients with bladder cancer – depending on tumor stage
title_full	Proteolytic parameter changes in the plasma of patients with bladder cancer – depending on tumor stage
title_fullStr	Proteolytic parameter changes in the plasma of patients with bladder cancer – depending on tumor stage
title_full_unstemmed	Proteolytic parameter changes in the plasma of patients with bladder cancer – depending on tumor stage
title_sort	proteolytic parameter changes in the plasma of patients with bladder cancer – depending on tumor stage
publisher	Sciendo
series	Current Issues in Pharmacy and Medical Sciences
issn	2084-980X 2300-6676
publishDate	2020-06-01
description	Bladder cancer (BC) is a worldwide common disease with a high mortality rate. Recognizing the dynamic changes in plasma that proteases and their inhibitors undergo might be valuable in understanding the carcinogenesis of invasive bladder cancer and in identifying BC patients with poor prognosis. This study aims to determine the activity of the proteolytic enzyme system and their inhibitors in patients with BC. In this paper, the total proteolytic activity, the activity of matrix metalloproteases (MMPs) and serine proteases was analyzed by the method of caseinolytic activity. For detection of activity of some inhibitors of proteolysis, we used the unified method for determining the activity of alpha-1-antitrypsin (α1A) and alpha-2-Macroglobulin (α2M) in human plasma. The level of medium-mass molecules (MMM) was assessed spectrophotometrically by applying the Nikolaichik method.
topic	bladder cancer serine proteases mmps α1a α2m medium mass molecules
url	https://doi.org/10.2478/cipms-2020-0013
work_keys_str_mv	AT dmytrykviktor proteolyticparameterchangesintheplasmaofpatientswithbladdercancerdependingontumorstage AT luhovskatetiana proteolyticparameterchangesintheplasmaofpatientswithbladdercancerdependingontumorstage AT yakovlevpavel proteolyticparameterchangesintheplasmaofpatientswithbladdercancerdependingontumorstage AT savchukolexiy proteolyticparameterchangesintheplasmaofpatientswithbladdercancerdependingontumorstage AT halenovatetiana proteolyticparameterchangesintheplasmaofpatientswithbladdercancerdependingontumorstage AT rakshanataliia proteolyticparameterchangesintheplasmaofpatientswithbladdercancerdependingontumorstage AT ostapchenkoludmila proteolyticparameterchangesintheplasmaofpatientswithbladdercancerdependingontumorstage
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Proteolytic parameter changes in the plasma of patients with bladder cancer – depending on tumor stage

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