The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells

The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells

Here, we developed a cell defined siRNA microarray (CDSM) for human bone marrow stromal cells (hBMSCs) designed to control the culture of cells inside the spot area without reducing the efficiency of siRNA silencing, “Development of a cell-defined siRNA microarray for analysis of gene functionin hum...

Full description

Bibliographic Details
Main Authors: Hi Chul Kim, Gi-Hwan Kim, David Shum, Ssang-Goo Cho, Eun Ju Lee, Yong-Jun Kwon
Format: Article
Language:English
Published: Elsevier 2016-06-01
Series:Data in Brief
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340916301482
id doaj-07a2a33485a74db9941e8a4f42ba3561
record_format Article
spelling doaj-07a2a33485a74db9941e8a4f42ba35612020-11-25T01:25:55ZengElsevierData in Brief2352-34092016-06-017673678The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cellsHi Chul Kim0Gi-Hwan Kim1David Shum2Ssang-Goo Cho3Eun Ju Lee4Yong-Jun Kwon5Institut Pasteur Korea, IP-Korea, 696 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do 463-400, Republic of Korea; Department of Animal Biotechnology (BK21), Animal Resources Research Center, Konkuk University, Seoul 143-702, Republic of KoreaBiomedical Research Institute & IRICT, Seoul National University Hospital, Seoul, Republic of KoreaInstitut Pasteur Korea, IP-Korea, 696 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do 463-400, Republic of KoreaDepartment of Animal Biotechnology (BK21), Animal Resources Research Center, Konkuk University, Seoul 143-702, Republic of KoreaBiomedical Research Institute & IRICT, Seoul National University Hospital, Seoul, Republic of Korea; Correspondence to: Biomedical Research Institute and IRICT, Center for Cell & Gene Therapy, Seoul National University Hospital, 101 Daehak ro, jongro gu, Seoul 110-744, Republic of Korea. Tel.: +82 2 2072 1707; fax: +82 2 742 8965.Institut Pasteur Korea, IP-Korea, 696 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do 463-400, Republic of Korea; Corresponding author. Current address: Ksilink, 16 Rue d’Ankara, Strasbourg 67000, France.Here, we developed a cell defined siRNA microarray (CDSM) for human bone marrow stromal cells (hBMSCs) designed to control the culture of cells inside the spot area without reducing the efficiency of siRNA silencing, “Development of a cell-defined siRNA microarray for analysis of gene functionin human bone marrow stromal cells” (Kim et al., 2016 [1]). First, we confirmed that p65 protein inhibition efficiency was maintained when hBMSCs were culture for 7 days on the siRNA spot, and siRNA spot activity remained in spite of long term storage (10 days and 2 months). Additionally, we confirmed p65 protein inhibition in U2OS cells after 48 h reverse transfection.http://www.sciencedirect.com/science/article/pii/S2352340916301482
collection DOAJ
language English
format Article
sources DOAJ
author Hi Chul Kim
Gi-Hwan Kim
David Shum
Ssang-Goo Cho
Eun Ju Lee
Yong-Jun Kwon
spellingShingle Hi Chul Kim
Gi-Hwan Kim
David Shum
Ssang-Goo Cho
Eun Ju Lee
Yong-Jun Kwon
The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells
Data in Brief
author_facet Hi Chul Kim
Gi-Hwan Kim
David Shum
Ssang-Goo Cho
Eun Ju Lee
Yong-Jun Kwon
author_sort Hi Chul Kim
title The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells
title_short The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells
title_full The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells
title_fullStr The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells
title_full_unstemmed The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells
title_sort monitoring of gene functions on a cell-defined sirna microarray in human bone marrow stromal and u2os cells
publisher Elsevier
series Data in Brief
issn 2352-3409
publishDate 2016-06-01
description Here, we developed a cell defined siRNA microarray (CDSM) for human bone marrow stromal cells (hBMSCs) designed to control the culture of cells inside the spot area without reducing the efficiency of siRNA silencing, “Development of a cell-defined siRNA microarray for analysis of gene functionin human bone marrow stromal cells” (Kim et al., 2016 [1]). First, we confirmed that p65 protein inhibition efficiency was maintained when hBMSCs were culture for 7 days on the siRNA spot, and siRNA spot activity remained in spite of long term storage (10 days and 2 months). Additionally, we confirmed p65 protein inhibition in U2OS cells after 48 h reverse transfection.
url http://www.sciencedirect.com/science/article/pii/S2352340916301482
work_keys_str_mv AT hichulkim themonitoringofgenefunctionsonacelldefinedsirnamicroarrayinhumanbonemarrowstromalandu2oscells
AT gihwankim themonitoringofgenefunctionsonacelldefinedsirnamicroarrayinhumanbonemarrowstromalandu2oscells
AT davidshum themonitoringofgenefunctionsonacelldefinedsirnamicroarrayinhumanbonemarrowstromalandu2oscells
AT ssanggoocho themonitoringofgenefunctionsonacelldefinedsirnamicroarrayinhumanbonemarrowstromalandu2oscells
AT eunjulee themonitoringofgenefunctionsonacelldefinedsirnamicroarrayinhumanbonemarrowstromalandu2oscells
AT yongjunkwon themonitoringofgenefunctionsonacelldefinedsirnamicroarrayinhumanbonemarrowstromalandu2oscells
AT hichulkim monitoringofgenefunctionsonacelldefinedsirnamicroarrayinhumanbonemarrowstromalandu2oscells
AT gihwankim monitoringofgenefunctionsonacelldefinedsirnamicroarrayinhumanbonemarrowstromalandu2oscells
AT davidshum monitoringofgenefunctionsonacelldefinedsirnamicroarrayinhumanbonemarrowstromalandu2oscells
AT ssanggoocho monitoringofgenefunctionsonacelldefinedsirnamicroarrayinhumanbonemarrowstromalandu2oscells
AT eunjulee monitoringofgenefunctionsonacelldefinedsirnamicroarrayinhumanbonemarrowstromalandu2oscells
AT yongjunkwon monitoringofgenefunctionsonacelldefinedsirnamicroarrayinhumanbonemarrowstromalandu2oscells
_version_ 1725111769595117568

Similar Items