Aggregation, Sedimentation, and Dissolution of Copper Oxide Nanoparticles: Influence of Low-Molecular-Weight Organic Acids from Root Exudates

• Main Authors: Cheng Peng, Hong Tong, Peng Yuan, Lijuan Sun, Lei Jiang, Jiyan Shi
• Format: Article
• Language: English
• Published: MDPI AG 2019-06-01
• Series: Nanomaterials
• Subjects: root exudates; low-molecular-weight organic acids; CuO nanoparticles; aggregation; dissolution
• Online Access: https://www.mdpi.com/2079-4991/9/6/841

The rhizosphere is an essential pathway for the uptake of metal-based nanoparticles (MNPs) by plant roots. However, the interaction between root exudates and MNPs is still unclear. In this study, we initially identified the major low-molecular-weight organic acids (LMWOAs) in the rice root exudates using hydroponics. Then, the individual LMWOAs were added to CuO nanoparticle suspensions to investigate their effects on the environmental behavior of the MNPs. The results showed that both the variety and the concentration of LMWOAs impacted the aggregation, sedimentation, and dissolution of CuO nanoparticles (NPs). Almost all LMWOAs except succinic acid inhibited the aggregation of CuO NPs by enhancing the electrostatic repulsive force between NPs. The presence of citric and oxalic acids rather than lactic acid greatly improved the stability of CuO NP suspensions, but other acids showed a low promoting and high inhibiting effect on NP sedimentation. Moreover, all the LMWOAs from root exudates facilitated the dissolution of CuO NPs with a positive dose-dependent correlation, especially formic acid. Notably, citric acid, as the most abundant LMWOAs in rice root exudates, largely determined the aggregation, sedimentation, and dissolution of CuO NPs. This study provides a better understanding on NP−plant interactions in the rhizosphere.