A New FRET-Based Sensitive DNA Sensor for Medical Diagnostics using PNA Probe and Water-Soluble Blue Light Emitting Polymer

A New FRET-Based Sensitive DNA Sensor for Medical Diagnostics using PNA Probe and Water-Soluble Blue Light Emitting Polymer

A reliable, fast, and low-cost biosensor for medical diagnostics using DNA sequence detection has been developed and tested for the detection of the bacterium “Bacillus anthracis.” In this sensor, Poly [9,9-di (6,6′- N, N′ trimethylammonium) hexylfluorenyl-2, 7-diyl)-alt-co- (1,4-phenylene)] dibromi...

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Main Authors: Nidhi Mathur, Anamika Aneja, P. K. Bhatnagar, P. C. Mathur
Format: Article
Language:English
Published: Hindawi Limited 2008-01-01
Series:Journal of Sensors
Online Access:http://dx.doi.org/10.1155/2008/270475
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spelling doaj-07763bc2be5b4acb8d629c0f8fcfdaed2020-11-24T23:54:51ZengHindawi LimitedJournal of Sensors1687-725X1687-72682008-01-01200810.1155/2008/270475270475A New FRET-Based Sensitive DNA Sensor for Medical Diagnostics using PNA Probe and Water-Soluble Blue Light Emitting PolymerNidhi Mathur0Anamika Aneja1P. K. Bhatnagar2P. C. Mathur3Department of Electronic Science, University of Delhi South Campus, Benito Juarez Road, New Delhi-110021, IndiaDepartment of Electronic Science, University of Delhi South Campus, Benito Juarez Road, New Delhi-110021, IndiaDepartment of Electronic Science, University of Delhi South Campus, Benito Juarez Road, New Delhi-110021, IndiaDepartment of Electronic Science, University of Delhi South Campus, Benito Juarez Road, New Delhi-110021, IndiaA reliable, fast, and low-cost biosensor for medical diagnostics using DNA sequence detection has been developed and tested for the detection of the bacterium “Bacillus anthracis.” In this sensor, Poly [9,9-di (6,6′- N, N′ trimethylammonium) hexylfluorenyl-2, 7-diyl)-alt-co- (1,4-phenylene)] dibromide salt (PFP) has been taken as cationic conjugated polymer (CCP) and PNA attached with fluorescein dye (PNAC∗) as a probe. The basic principle of this sensor is that when a PNAC∗ probe is hybridized with a single strand DNA (ssDNA) having complementary sequence, Forster resonance energy transfer (FRET) may take place from PFP to the PNAC∗/DNA complex. If the FRET is efficient, the photoluminescence from the PFP will be highly quenched and that from PNAC∗ will be enhanced. On the other hand, if the DNA sequence is noncomplementary to PNA, FRET will not occur.http://dx.doi.org/10.1155/2008/270475
collection DOAJ
language English
format Article
sources DOAJ
author Nidhi Mathur
Anamika Aneja
P. K. Bhatnagar
P. C. Mathur
spellingShingle Nidhi Mathur
Anamika Aneja
P. K. Bhatnagar
P. C. Mathur
A New FRET-Based Sensitive DNA Sensor for Medical Diagnostics using PNA Probe and Water-Soluble Blue Light Emitting Polymer
Journal of Sensors
author_facet Nidhi Mathur
Anamika Aneja
P. K. Bhatnagar
P. C. Mathur
author_sort Nidhi Mathur
title A New FRET-Based Sensitive DNA Sensor for Medical Diagnostics using PNA Probe and Water-Soluble Blue Light Emitting Polymer
title_short A New FRET-Based Sensitive DNA Sensor for Medical Diagnostics using PNA Probe and Water-Soluble Blue Light Emitting Polymer
title_full A New FRET-Based Sensitive DNA Sensor for Medical Diagnostics using PNA Probe and Water-Soluble Blue Light Emitting Polymer
title_fullStr A New FRET-Based Sensitive DNA Sensor for Medical Diagnostics using PNA Probe and Water-Soluble Blue Light Emitting Polymer
title_full_unstemmed A New FRET-Based Sensitive DNA Sensor for Medical Diagnostics using PNA Probe and Water-Soluble Blue Light Emitting Polymer
title_sort new fret-based sensitive dna sensor for medical diagnostics using pna probe and water-soluble blue light emitting polymer
publisher Hindawi Limited
series Journal of Sensors
issn 1687-725X
1687-7268
publishDate 2008-01-01
description A reliable, fast, and low-cost biosensor for medical diagnostics using DNA sequence detection has been developed and tested for the detection of the bacterium “Bacillus anthracis.” In this sensor, Poly [9,9-di (6,6′- N, N′ trimethylammonium) hexylfluorenyl-2, 7-diyl)-alt-co- (1,4-phenylene)] dibromide salt (PFP) has been taken as cationic conjugated polymer (CCP) and PNA attached with fluorescein dye (PNAC∗) as a probe. The basic principle of this sensor is that when a PNAC∗ probe is hybridized with a single strand DNA (ssDNA) having complementary sequence, Forster resonance energy transfer (FRET) may take place from PFP to the PNAC∗/DNA complex. If the FRET is efficient, the photoluminescence from the PFP will be highly quenched and that from PNAC∗ will be enhanced. On the other hand, if the DNA sequence is noncomplementary to PNA, FRET will not occur.
url http://dx.doi.org/10.1155/2008/270475
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