PARTIAL PURIFICATION AND IMMUNO-BIOCHEMICAL CHARACTERISATION OF FERTILITY ASSOCIATED PROTEIN OF KARAN FRIES BULL SEMINAL PLASMA

• Main Authors: Brajesh Raman, Subhasis Batabyal, Saibal Chattopadhyay, S S Kesh, Abhijit Barui
• Format: Article
• Language: English
• Published: West Bengal Veterinary Alumni Association 2014-06-01
• Series: Exploratory Animal and Medical Research
• Subjects: Karan Fries Bull; Seminal Plasma; 26 kDa; Fertility associated Protein; Immunodominant Protein
• Online Access: http://www.animalmedicalresearch.org/Vol.4_Issue-1_June_2014/Brajesh%20Raman.pdf

The objective of the present study was detection, isolation, partial purification and immunobiochemical characterization of fertility associated protein in the seminal plasma of high prolific Karan fries bull. Seminal plasma of Karan Fries bull was partially purified by gel filtration chromatography and analyzed by 10% SDS-PAGE for their polypeptide profile. PAGE analysis revealed major band of 55 kDa, and 26 kDa. Hyperimmune serum was raised in rabbit against crude seminal plasma protein. Single precipitin line was observed in DID test when each of the partially purified 26 kDa and 55 kDa proteins were reacted with hyperimmune serum. These proteins were also found to be immunoreactive against hyperimmune serum in Western blot technique.