PARTIAL PURIFICATION AND IMMUNO-BIOCHEMICAL CHARACTERISATION OF FERTILITY ASSOCIATED PROTEIN OF KARAN FRIES BULL SEMINAL PLASMA
The objective of the present study was detection, isolation, partial purification and immunobiochemical characterization of fertility associated protein in the seminal plasma of high prolific Karan fries bull. Seminal plasma of Karan Fries bull was partially purified by gel filtration chromatogra...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
West Bengal Veterinary Alumni Association
2014-06-01
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| Series: | Exploratory Animal and Medical Research |
| Subjects: | |
| Online Access: | http://www.animalmedicalresearch.org/Vol.4_Issue-1_June_2014/Brajesh%20Raman.pdf |
| Summary: | The objective of the present study was detection, isolation, partial purification and
immunobiochemical characterization of fertility associated protein in the seminal plasma of high
prolific Karan fries bull. Seminal plasma of Karan Fries bull was partially purified by gel filtration
chromatography and analyzed by 10% SDS-PAGE for their polypeptide profile. PAGE analysis
revealed major band of 55 kDa, and 26 kDa. Hyperimmune serum was raised in rabbit against crude
seminal plasma protein. Single precipitin line was observed in DID test when each of the partially
purified 26 kDa and 55 kDa proteins were reacted with hyperimmune serum. These proteins were
also found to be immunoreactive against hyperimmune serum in Western blot technique. |
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| ISSN: | 2277-470X 2319-247X |