An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film
A new amperometric uric acid biosensor was developed by immobilizing uricase by a glutaraldehyde crosslinking procedure on polyaniline-polypyrrole (pani-ppy) composite film on the surface of a platinum electrode. Determination of uric acid was performed by the oxidation of enzymatically generated H2...
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MDPI AG
2008-09-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/8/9/5492/ |
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doaj-07452f66e0e44dada32fed1cf9756f922020-11-24T22:24:41ZengMDPI AGSensors1424-82202008-09-01895492550010.3390/s8095492An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole FilmFatma ArslanA new amperometric uric acid biosensor was developed by immobilizing uricase by a glutaraldehyde crosslinking procedure on polyaniline-polypyrrole (pani-ppy) composite film on the surface of a platinum electrode. Determination of uric acid was performed by the oxidation of enzymatically generated H2O2 at 0.4 V vs. Ag/AgCl. The linear working range of the biosensor was 2.5×10-6 – 8.5×10-5 M and the response time was about 70 s. The effects of pH, temperature were investigated and optimum parameters were found to be 9.0, 55 oC, respectively. The stability and reproducibility of the enzyme electrode have been also studied.http://www.mdpi.com/1424-8220/8/9/5492/Uric acidUricaseBiosensorPolypyrrolePolyaniline |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Fatma Arslan |
| spellingShingle |
Fatma Arslan An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film Sensors Uric acid Uricase Biosensor Polypyrrole Polyaniline |
| author_facet |
Fatma Arslan |
| author_sort |
Fatma Arslan |
| title |
An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film |
| title_short |
An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film |
| title_full |
An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film |
| title_fullStr |
An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film |
| title_full_unstemmed |
An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film |
| title_sort |
amperometric biosensor for uric acid determination prepared from uricase immobilized in polyaniline-polypyrrole film |
| publisher |
MDPI AG |
| series |
Sensors |
| issn |
1424-8220 |
| publishDate |
2008-09-01 |
| description |
A new amperometric uric acid biosensor was developed by immobilizing uricase by a glutaraldehyde crosslinking procedure on polyaniline-polypyrrole (pani-ppy) composite film on the surface of a platinum electrode. Determination of uric acid was performed by the oxidation of enzymatically generated H2O2 at 0.4 V vs. Ag/AgCl. The linear working range of the biosensor was 2.5×10-6 – 8.5×10-5 M and the response time was about 70 s. The effects of pH, temperature were investigated and optimum parameters were found to be 9.0, 55 oC, respectively. The stability and reproducibility of the enzyme electrode have been also studied. |
| topic |
Uric acid Uricase Biosensor Polypyrrole Polyaniline |
| url |
http://www.mdpi.com/1424-8220/8/9/5492/ |
| work_keys_str_mv |
AT fatmaarslan anamperometricbiosensorforuricaciddeterminationpreparedfromuricaseimmobilizedinpolyanilinepolypyrrolefilm AT fatmaarslan amperometricbiosensorforuricaciddeterminationpreparedfromuricaseimmobilizedinpolyanilinepolypyrrolefilm |
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