5-Aza-CdR Regulates RASSF1A By Inhibiting DNMT1 To Affect Colon Cancer Cell Proliferation, Migration And Apoptosis

• Main Authors: Chen J, Wu L, Xu H, Cheng S
• Format: Article
• Language: English
• Published: Dove Medical Press 2019-11-01
• Series: Cancer Management and Research
• Subjects: dna methylation; colon cancer; rassf1a; 5-aza-cdr; dnmt1
• Online Access: https://www.dovepress.com/5-aza-cdr-regulates-rassf1a-by-inhibiting-dnmt1-to-affect-colon-cancer-peer-reviewed-article-CMAR

Jinyuan Chen,1 Lixiang Wu,2 Hong Xu,3 Shubang Cheng1 1Department of Gastrointestinal Surgery, People’s Hospital of Longhua, Shenzhen, Guangdong 518109, People’s Republic of China; 2Guangzhou Concord Cancer Center, Guangzhou, Guangdong 510620, People’s Republic of China; 3Department of Pharmacy, People’s Hospital of Longhua, Shenzhen, Guangdong 518109, People’s Republic of ChinaCorrespondence: Shubang ChengDepartment of Gastrointestinal Surgery, People’s Hospital of Longhua, Shenzhen, Guangdong 518109, People’s Republic of ChinaEmail [e-mail]: To evaluate 5-Aza-CdR’s inhibited effects on migration, proliferation, and apoptosis in colon cancer cells and its potential mechanisms.Methods: HCT-116, SW480, and SW620 were divided into HCT116 group, HCT116+5-Aza-CdR group, SW480 group, SW480+5-Aza-CdR group, SW620 group and SW620+5-Aza according to experimental needs. MTT test was chosen to investigate cell proliferation; Transwell test was used to evaluate cell migration; scratch assay was used to investigate cell invasion; flow cytometry was used to investigate apoptosis; immunofluorescence assay was used to investigate the protein level of DNMT1 and RASSF1A in cells; qRT-PCR was used to examine DNMT1, RASSF1A, RAS, Raf1, MEK, Grb2 and ERK transcription levels.Results: Compared with HCT116 group, 5-Aza-CdR+HCT116 group inhibited cell proliferation, increased apoptosis rate, decreased invasive ability, decreased DNMT1 expression, increased expression of RASSF1A, decreased expression of RAS, Raf1, MEK, Grb2 and ERK. SW480 was compared with 5-Aza-CdR+SW480 group and SW620 group with 5-Aza-CdR+SW620 group. Their change trend of detection index was similar to that in HCT-116 group and HCT116+5-Aza-CdR group.Conclusion: 5-Aza-CdR can obviously inhibit the proliferation, migration and invasion of three colon cancer cell lines. Its mechanism maybe relies on the inhibition of DNMT1 mRNA level and protein level and the enhancement of RASSF1A mRNA level and protein level.Keywords: DNA methylation, colon cancer, RASSF1A, 5-Aza-CdR, DNMT1