Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60
Protective action of melatonin on human peripheral blood mononuclear cells exposed to gamma radiation Co60. Objective: toevaluate the protective effect of melatonin on peripheral blood mononuclear cells (PBMCs) exposed in vitro to ionizing radiation. Materialsand methods: PBMCs drawn from healthy vo...
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Pontificia Universidad Javeriana
2009-04-01
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Online Access: | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1398/860 |
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doaj-06faed05b79b4e878ce4f2aa09656c382020-11-25T02:09:27ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522009-04-011414147Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60Martha Mesa-VillanuevaMagaly Rueda-MartínezRuby Zayas-CamachoDiana Sicard-SuárezProtective action of melatonin on human peripheral blood mononuclear cells exposed to gamma radiation Co60. Objective: toevaluate the protective effect of melatonin on peripheral blood mononuclear cells (PBMCs) exposed in vitro to ionizing radiation. Materialsand methods: PBMCs drawn from healthy volunteers were incubated with 0, 1x10-5, 1x10-6 y 1x10-7 M melatonin for 10 minutes beforebeing exposed to gamma radiation (300 cGy; Co60 source). Afterwards, DNA damage was evaluated with the comet assay. Results:PBMCs pretreated with melatonin showed comet tails shorter than those without the hormone treatment, as well as a lower percentage ofcells with severe DNA damage. Conclusion: melatonin doses of 1x10-5, 1x10-6 and 1x10-7 M provide in vitro protection to PBMCs fromDNA damage (single strand breaks and alkali-labile sites) induced by gamma radiation (300 cGy; Co60 source).http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1398/860blood mononuclear cellscomet assayDNA damagegamma radiationmelatonin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Martha Mesa-Villanueva Magaly Rueda-Martínez Ruby Zayas-Camacho Diana Sicard-Suárez |
spellingShingle |
Martha Mesa-Villanueva Magaly Rueda-Martínez Ruby Zayas-Camacho Diana Sicard-Suárez Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60 Universitas Scientiarum blood mononuclear cells comet assay DNA damage gamma radiation melatonin |
author_facet |
Martha Mesa-Villanueva Magaly Rueda-Martínez Ruby Zayas-Camacho Diana Sicard-Suárez |
author_sort |
Martha Mesa-Villanueva |
title |
Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60 |
title_short |
Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60 |
title_full |
Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60 |
title_fullStr |
Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60 |
title_full_unstemmed |
Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60 |
title_sort |
acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma co60 |
publisher |
Pontificia Universidad Javeriana |
series |
Universitas Scientiarum |
issn |
0122-7483 2027-1352 |
publishDate |
2009-04-01 |
description |
Protective action of melatonin on human peripheral blood mononuclear cells exposed to gamma radiation Co60. Objective: toevaluate the protective effect of melatonin on peripheral blood mononuclear cells (PBMCs) exposed in vitro to ionizing radiation. Materialsand methods: PBMCs drawn from healthy volunteers were incubated with 0, 1x10-5, 1x10-6 y 1x10-7 M melatonin for 10 minutes beforebeing exposed to gamma radiation (300 cGy; Co60 source). Afterwards, DNA damage was evaluated with the comet assay. Results:PBMCs pretreated with melatonin showed comet tails shorter than those without the hormone treatment, as well as a lower percentage ofcells with severe DNA damage. Conclusion: melatonin doses of 1x10-5, 1x10-6 and 1x10-7 M provide in vitro protection to PBMCs fromDNA damage (single strand breaks and alkali-labile sites) induced by gamma radiation (300 cGy; Co60 source). |
topic |
blood mononuclear cells comet assay DNA damage gamma radiation melatonin |
url |
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1398/860 |
work_keys_str_mv |
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