Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60

Protective action of melatonin on human peripheral blood mononuclear cells exposed to gamma radiation Co60. Objective: toevaluate the protective effect of melatonin on peripheral blood mononuclear cells (PBMCs) exposed in vitro to ionizing radiation. Materialsand methods: PBMCs drawn from healthy vo...

Full description

Bibliographic Details
Main Authors: Martha Mesa-Villanueva, Magaly Rueda-Martínez, Ruby Zayas-Camacho, Diana Sicard-Suárez
Format: Article
Language:English
Published: Pontificia Universidad Javeriana 2009-04-01
Series:Universitas Scientiarum
Subjects:
Online Access:http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1398/860
id doaj-06faed05b79b4e878ce4f2aa09656c38
record_format Article
spelling doaj-06faed05b79b4e878ce4f2aa09656c382020-11-25T02:09:27ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522009-04-011414147Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60Martha Mesa-VillanuevaMagaly Rueda-MartínezRuby Zayas-CamachoDiana Sicard-SuárezProtective action of melatonin on human peripheral blood mononuclear cells exposed to gamma radiation Co60. Objective: toevaluate the protective effect of melatonin on peripheral blood mononuclear cells (PBMCs) exposed in vitro to ionizing radiation. Materialsand methods: PBMCs drawn from healthy volunteers were incubated with 0, 1x10-5, 1x10-6 y 1x10-7 M melatonin for 10 minutes beforebeing exposed to gamma radiation (300 cGy; Co60 source). Afterwards, DNA damage was evaluated with the comet assay. Results:PBMCs pretreated with melatonin showed comet tails shorter than those without the hormone treatment, as well as a lower percentage ofcells with severe DNA damage. Conclusion: melatonin doses of 1x10-5, 1x10-6 and 1x10-7 M provide in vitro protection to PBMCs fromDNA damage (single strand breaks and alkali-labile sites) induced by gamma radiation (300 cGy; Co60 source).http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1398/860blood mononuclear cellscomet assayDNA damagegamma radiationmelatonin
collection DOAJ
language English
format Article
sources DOAJ
author Martha Mesa-Villanueva
Magaly Rueda-Martínez
Ruby Zayas-Camacho
Diana Sicard-Suárez
spellingShingle Martha Mesa-Villanueva
Magaly Rueda-Martínez
Ruby Zayas-Camacho
Diana Sicard-Suárez
Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60
Universitas Scientiarum
blood mononuclear cells
comet assay
DNA damage
gamma radiation
melatonin
author_facet Martha Mesa-Villanueva
Magaly Rueda-Martínez
Ruby Zayas-Camacho
Diana Sicard-Suárez
author_sort Martha Mesa-Villanueva
title Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60
title_short Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60
title_full Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60
title_fullStr Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60
title_full_unstemmed Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60
title_sort acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma co60
publisher Pontificia Universidad Javeriana
series Universitas Scientiarum
issn 0122-7483
2027-1352
publishDate 2009-04-01
description Protective action of melatonin on human peripheral blood mononuclear cells exposed to gamma radiation Co60. Objective: toevaluate the protective effect of melatonin on peripheral blood mononuclear cells (PBMCs) exposed in vitro to ionizing radiation. Materialsand methods: PBMCs drawn from healthy volunteers were incubated with 0, 1x10-5, 1x10-6 y 1x10-7 M melatonin for 10 minutes beforebeing exposed to gamma radiation (300 cGy; Co60 source). Afterwards, DNA damage was evaluated with the comet assay. Results:PBMCs pretreated with melatonin showed comet tails shorter than those without the hormone treatment, as well as a lower percentage ofcells with severe DNA damage. Conclusion: melatonin doses of 1x10-5, 1x10-6 and 1x10-7 M provide in vitro protection to PBMCs fromDNA damage (single strand breaks and alkali-labile sites) induced by gamma radiation (300 cGy; Co60 source).
topic blood mononuclear cells
comet assay
DNA damage
gamma radiation
melatonin
url http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1398/860
work_keys_str_mv AT marthamesavillanueva accionprotectoradelamelatoninasobrecelulasmononuclearesdesangreperifericahumanasometidasaradiaciongammaco60
AT magalyruedamartinez accionprotectoradelamelatoninasobrecelulasmononuclearesdesangreperifericahumanasometidasaradiaciongammaco60
AT rubyzayascamacho accionprotectoradelamelatoninasobrecelulasmononuclearesdesangreperifericahumanasometidasaradiaciongammaco60
AT dianasicardsuarez accionprotectoradelamelatoninasobrecelulasmononuclearesdesangreperifericahumanasometidasaradiaciongammaco60
_version_ 1724923700081328128