Effect of aflatoxin B1 on growth and enzymatic activity of a native strain of Bacillus sp Efecto de la aflatoxina B1 sobre el crecimiento y actividad proteolítica de una cepa nativa de Bacillus sp

The effect of different aflatoxin B1 (AFAB1) concentrations on alkaline protease growth and enzymatic activity was evaluated; a native strain of alkalophilic Bacillus sp cultivated in CSL (Corn Steep Liquor) was used. It was found that the effect of AFAB1 on the strain inhibited its growth and enzym...

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Bibliographic Details
Main Authors: Márquez Edna Judith, Montoya Olga, Sáez Vega Alex
Format: Article
Language:Spanish
Published: Universidad Nacional de Colombia 2004-07-01
Series:Revista Colombiana de Biotecnología
Subjects:
Online Access:http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/543
Description
Summary:The effect of different aflatoxin B1 (AFAB1) concentrations on alkaline protease growth and enzymatic activity was evaluated; a native strain of alkalophilic Bacillus sp cultivated in CSL (Corn Steep Liquor) was used. It was found that the effect of AFAB1 on the strain inhibited its growth and enzymatic activity to 1 ppm, showing that the strain is highly sensible to AFAB1, meaning that medium obtained f rom Colombian corn contaminated with this mycotoxin cannot be easily used. Concentrations less than 0.1 ppm did not affect growth and enzymatic activity.&#13; &#13; Key words: Bacillus, aflatoxin, alkaline proteases.<br>Se evaluó el efecto de diferentes concentraciones de aflatoxina B1 (AFAB1) sobre el crecimiento y actividad enzimática de proteasas alcalinas de una cepa nativa de Bacillus sp Alcalofílico cultivada en LAM (Licor Agotado de Maíz). Se encontró que la cepa inhibe su crecimiento y actividad enzimática a 1 ppm, lo que demuestra una alta sensibilidad de la cepa evaluada a la AFAB1 e imposibilita utilizar fácilmente medios obtenidos de maíz nacional contaminado con esta micotoxina. Las concentraciones inferiores a 0.1 ppm no tienen ningún efecto sobre el crecimiento y la actividad enzimática.&#13; &#13; Palabras clave: Bacillus, aflatoxina, proteasas alcalinas.
ISSN:0123-3475
1909-8758