A method to measure hydrolytic activity of adenosinetriphosphatases (ATPases).

The detection of small amounts (nanomoles) of inorganic phosphate has a great interest in biochemistry. In particular, phosphate detection is useful to evaluate the rate of hydrolysis of phosphatases, that are enzymes able to remove phosphate from their substrate by hydrolytic cleavage. The hydrolys...

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Bibliographic Details
Main Authors: Gianluca Bartolommei, Maria Rosa Moncelli, Francesco Tadini-Buoninsegni
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3589382?pdf=render