An Isocratic Toxic Chemical-Free Mobile Phase HPLC-PDA Analysis of Malachite Green and Leuco-Malachite Green
This paper describes a reserved-phase high-performance liquid chromatographic (HPLC) method for detecting malachite green (MG) and leuco-malachite green (LMG) using an isocratic toxic organic solvent/reagent-free mobile phase. Chromatographic separations were performed an Inertsil® WP300 C4 with 0.0...
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MDPI AG
2014-05-01
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| Series: | Chromatography |
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| Online Access: | http://www.mdpi.com/2227-9075/1/2/75 |
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doaj-069852793eed4ec983defe6b763e56542020-11-25T01:30:15ZengMDPI AGChromatography2227-90752014-05-0112758110.3390/chromatography1020075chromatography1020075An Isocratic Toxic Chemical-Free Mobile Phase HPLC-PDA Analysis of Malachite Green and Leuco-Malachite GreenNaoto Furusawa0Graduate School of Human Life Science, Osaka City University, Osaka 558-8585, JapanThis paper describes a reserved-phase high-performance liquid chromatographic (HPLC) method for detecting malachite green (MG) and leuco-malachite green (LMG) using an isocratic toxic organic solvent/reagent-free mobile phase. Chromatographic separations were performed an Inertsil® WP300 C4 with 0.02 mol/L octane sulfonic acid–ethanol mobile phase and a photodiode-array detector. The total run time was <5 min. The system suitability was well within the international acceptance criteria. A harmless method for simultaneously detecting MG and LMG was developed and may be further applied to the quantification in foods.http://www.mdpi.com/2227-9075/1/2/75internal harmonized analytical methodmalachite greenleuco-malachite greenhigh-performance liquid chromatographyphoto-diode array |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Naoto Furusawa |
| spellingShingle |
Naoto Furusawa An Isocratic Toxic Chemical-Free Mobile Phase HPLC-PDA Analysis of Malachite Green and Leuco-Malachite Green Chromatography internal harmonized analytical method malachite green leuco-malachite green high-performance liquid chromatography photo-diode array |
| author_facet |
Naoto Furusawa |
| author_sort |
Naoto Furusawa |
| title |
An Isocratic Toxic Chemical-Free Mobile Phase HPLC-PDA Analysis of Malachite Green and Leuco-Malachite Green |
| title_short |
An Isocratic Toxic Chemical-Free Mobile Phase HPLC-PDA Analysis of Malachite Green and Leuco-Malachite Green |
| title_full |
An Isocratic Toxic Chemical-Free Mobile Phase HPLC-PDA Analysis of Malachite Green and Leuco-Malachite Green |
| title_fullStr |
An Isocratic Toxic Chemical-Free Mobile Phase HPLC-PDA Analysis of Malachite Green and Leuco-Malachite Green |
| title_full_unstemmed |
An Isocratic Toxic Chemical-Free Mobile Phase HPLC-PDA Analysis of Malachite Green and Leuco-Malachite Green |
| title_sort |
isocratic toxic chemical-free mobile phase hplc-pda analysis of malachite green and leuco-malachite green |
| publisher |
MDPI AG |
| series |
Chromatography |
| issn |
2227-9075 |
| publishDate |
2014-05-01 |
| description |
This paper describes a reserved-phase high-performance liquid chromatographic (HPLC) method for detecting malachite green (MG) and leuco-malachite green (LMG) using an isocratic toxic organic solvent/reagent-free mobile phase. Chromatographic separations were performed an Inertsil® WP300 C4 with 0.02 mol/L octane sulfonic acid–ethanol mobile phase and a photodiode-array detector. The total run time was <5 min. The system suitability was well within the international acceptance criteria. A harmless method for simultaneously detecting MG and LMG was developed and may be further applied to the quantification in foods. |
| topic |
internal harmonized analytical method malachite green leuco-malachite green high-performance liquid chromatography photo-diode array |
| url |
http://www.mdpi.com/2227-9075/1/2/75 |
| work_keys_str_mv |
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