Varicella Zoster Virus and Large Vessel Vasculitis, the Absence of an Association
Objective: It is controversial whether microorganisms play a role in the pathogenesis of large and medium vessel vasculitides (eg, giant cell arteritis [GCA], Takayasu arteritis [TAK] and focal idiopathic aortitis [FIA]). Recent studies have reported the presence of Varicella Zoster Virus (VZV) with...
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Case Western Reserve University
2017-06-01
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| Series: | Pathogens and Immunity |
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| Online Access: | https://paijournal.com/index.php/paijournal/article/view/196 |
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doaj-0677ac503dc84e1ab63b8ea4c08d15cd2020-11-24T23:53:18ZengCase Western Reserve UniversityPathogens and Immunity2469-29642017-06-012222823810.20411/pai.v2i2.19657Varicella Zoster Virus and Large Vessel Vasculitis, the Absence of an AssociationGary W. Procop0Charis Eng1Alison Clifford2Alexandra Villa-Forte3Leonard H. Calabrese4Eric Roselli5Lars Svensson6Douglas Johnston7Gosta Pettersson8Edward Soltesz9Lisa Lystad10Julian D. Perry11Alexander Blandford12Deborah A. Wilson13Gary S. Hoffman14Pathology and Laboratory Medicine Institute, Cleveland Clinic, Cleveland, OhioGenomic Medicine Institute, Lerner Research Institute, Taussig Cancer Institute, Cleveland ClinicCenter for Vasculitis Care and Research, Department of Rheumatic and Immunologic Diseases, Cleveland Clinic, Cleveland, Ohio; Division of Rheumatology, University of Alberta, CanadaCenter for Vasculitis Care and Research, Department of Rheumatic and Immunologic Diseases, Cleveland Clinic, Cleveland, OhioCenter for Vasculitis Care and Research, Department of Rheumatic and Immunologic Diseases, Cleveland Clinic, Cleveland, OhioHeart & Vascular Institute, Cleveland Clinic, Cleveland, OhioHeart & Vascular Institute, Cleveland Clinic, Cleveland, OhioHeart & Vascular Institute, Cleveland Clinic, Cleveland, OhioHeart & Vascular Institute, Cleveland Clinic, Cleveland, OhioHeart & Vascular Institute, Cleveland Clinic, Cleveland, OhioCole Eye Institute, Cleveland Clinic, Cleveland, OhioCole Eye Institute, Cleveland Clinic, Cleveland, OhioCole Eye Institute, Cleveland Clinic, Cleveland, OhioPathology and Laboratory Medicine Institute, Cleveland Clinic, Cleveland, OhioCenter for Vasculitis Care and Research, Department of Rheumatic and Immunologic Diseases, Cleveland Clinic, Cleveland, OhioObjective: It is controversial whether microorganisms play a role in the pathogenesis of large and medium vessel vasculitides (eg, giant cell arteritis [GCA], Takayasu arteritis [TAK] and focal idiopathic aortitis [FIA]). Recent studies have reported the presence of Varicella Zoster Virus (VZV) within formalin-fixed, paraffin-embedded temporal arteries and aortas of about three-quarters or more of patients with these conditions, and in a minority of controls. In a prospective study, we sought to confirm these findings using DNA extracted from vessels that were harvested under surgically aseptic conditions and snap frozen. Methods and Results: DNA samples extracted from 11 surgically sterile temporal arteries and 31 surgically sterile thoracic aortas were used in an attempt to identify the vessel-associated VZV genome. Two different validated PCR methods were used. Thirty-one thoracic aorta aneurysm specimens included biopsies from 8 patients with GCA, 2 from patients with TAK, 6 from patients with FIA, and 15 from patients without vasculitis, who had non-inflammatory aneurysms. Eleven temporal artery biopsies were collected from 5 patients with GCA and 6 controls. The presence of VZV was not identified in either the specimens from patients with large vessel vasculitis or from the controls. Conclusions: Using surgically sterile snap-frozen specimens, we were unable to confirm recent reports of the presence of VZV in either aortas or temporal arteries from patients with large vessel vasculitis or controls. Keywords: Aorta and temporal artery biopsies, Varicella Zoster Virus, Large Vessel Vasculitishttps://paijournal.com/index.php/paijournal/article/view/196Aorta and temporal artery biopsies, Varicella Zoster Virus, Large Vessel Vasculitis |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Gary W. Procop Charis Eng Alison Clifford Alexandra Villa-Forte Leonard H. Calabrese Eric Roselli Lars Svensson Douglas Johnston Gosta Pettersson Edward Soltesz Lisa Lystad Julian D. Perry Alexander Blandford Deborah A. Wilson Gary S. Hoffman |
| spellingShingle |
Gary W. Procop Charis Eng Alison Clifford Alexandra Villa-Forte Leonard H. Calabrese Eric Roselli Lars Svensson Douglas Johnston Gosta Pettersson Edward Soltesz Lisa Lystad Julian D. Perry Alexander Blandford Deborah A. Wilson Gary S. Hoffman Varicella Zoster Virus and Large Vessel Vasculitis, the Absence of an Association Pathogens and Immunity Aorta and temporal artery biopsies, Varicella Zoster Virus, Large Vessel Vasculitis |
| author_facet |
Gary W. Procop Charis Eng Alison Clifford Alexandra Villa-Forte Leonard H. Calabrese Eric Roselli Lars Svensson Douglas Johnston Gosta Pettersson Edward Soltesz Lisa Lystad Julian D. Perry Alexander Blandford Deborah A. Wilson Gary S. Hoffman |
| author_sort |
Gary W. Procop |
| title |
Varicella Zoster Virus and Large Vessel Vasculitis, the Absence of an Association |
| title_short |
Varicella Zoster Virus and Large Vessel Vasculitis, the Absence of an Association |
| title_full |
Varicella Zoster Virus and Large Vessel Vasculitis, the Absence of an Association |
| title_fullStr |
Varicella Zoster Virus and Large Vessel Vasculitis, the Absence of an Association |
| title_full_unstemmed |
Varicella Zoster Virus and Large Vessel Vasculitis, the Absence of an Association |
| title_sort |
varicella zoster virus and large vessel vasculitis, the absence of an association |
| publisher |
Case Western Reserve University |
| series |
Pathogens and Immunity |
| issn |
2469-2964 |
| publishDate |
2017-06-01 |
| description |
Objective: It is controversial whether microorganisms play a role in the pathogenesis of large and medium vessel vasculitides (eg, giant cell arteritis [GCA], Takayasu arteritis [TAK] and focal idiopathic aortitis [FIA]). Recent studies have reported the presence of Varicella Zoster Virus (VZV) within formalin-fixed, paraffin-embedded temporal arteries and aortas of about three-quarters or more of patients with these conditions, and in a minority of controls. In a prospective study, we sought to confirm these findings using DNA extracted from vessels that were harvested under surgically aseptic conditions and snap frozen.
Methods and Results: DNA samples extracted from 11 surgically sterile temporal arteries and 31 surgically sterile thoracic aortas were used in an attempt to identify the vessel-associated VZV genome. Two different validated PCR methods were used. Thirty-one thoracic aorta aneurysm specimens included biopsies from 8 patients with GCA, 2 from patients with TAK, 6 from patients with FIA, and 15 from patients without vasculitis, who had non-inflammatory aneurysms. Eleven temporal artery biopsies were collected from 5 patients with GCA and 6 controls. The presence of VZV was not identified in either the specimens from patients with large vessel vasculitis or from the controls.
Conclusions: Using surgically sterile snap-frozen specimens, we were unable to confirm recent reports of the presence of VZV in either aortas or temporal arteries from patients with large vessel vasculitis or controls.
Keywords: Aorta and temporal artery biopsies, Varicella Zoster Virus, Large Vessel Vasculitis |
| topic |
Aorta and temporal artery biopsies, Varicella Zoster Virus, Large Vessel Vasculitis |
| url |
https://paijournal.com/index.php/paijournal/article/view/196 |
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