Molecular analysis of Pseudomonas aeruginosa strains isolated from cystic fibrosis patients

Abstract Pseudomonas aeruginosa is a severe bacterial pathogen. Due to the genetic flexibility among strains, chronic airways infection can lead to mortality among cystic fibrosis (CF) patients. It is essential to develop patient-specific therapy which will rely on phenotypic and genomic diversity....

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Main Authors: Dariusz Jarych, Ewa Augustynowicz-Kopec, Agnieszka Iwanska, Pawel Parniewski, Marta Majchrzak
Format: Article
Language:English
Published: Nature Publishing Group 2021-07-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-95034-2
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spelling doaj-0608a96347a342b6b9538913b0ad75682021-08-01T11:25:29ZengNature Publishing GroupScientific Reports2045-23222021-07-011111910.1038/s41598-021-95034-2Molecular analysis of Pseudomonas aeruginosa strains isolated from cystic fibrosis patientsDariusz Jarych0Ewa Augustynowicz-Kopec1Agnieszka Iwanska2Pawel Parniewski3Marta Majchrzak4Institute of Medical Biology PASDepartment of Microbiology, National Tuberculosis and Lung Diseases Research InstituteDepartment of Microbiology, National Tuberculosis and Lung Diseases Research InstituteInstitute of Medical Biology PASInstitute of Medical Biology PASAbstract Pseudomonas aeruginosa is a severe bacterial pathogen. Due to the genetic flexibility among strains, chronic airways infection can lead to mortality among cystic fibrosis (CF) patients. It is essential to develop patient-specific therapy which will rely on phenotypic and genomic diversity. The primary objective of this study was to assess the genomic variability of P. aeruginosa strains, using two different molecular techniques for tracking the epidemiological transmissions. This study applied a multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) for an efficient genotyping of clinical P. aeruginosa strains isolated from CF patients and compared results with a TRS-PCR typing. The percentage similarity analysis was performed using the categorical multi-state coefficient and UPGMA method. Based on the MLVA and TRS-PCR group assessment, 43 P. aeruginosa strains/variants were detected among the 63 clinical isolates from eight CF patients. The study of P. aeruginosa isolates has revealed that during chronic bacterial infections, CF patients harbor different P. aeruginosa strains or variants within the same host over the years. P. aeruginosa genotypes diversity may result from infection with several strains and result from a microevolution process of an initially acquired strain. The TRS-PCR method proposed in this work can complement the MLVA scheme. It can also be used as a preliminary method for genetic typing of P. aeruginosa isolates in CF patients.https://doi.org/10.1038/s41598-021-95034-2
collection DOAJ
language English
format Article
sources DOAJ
author Dariusz Jarych
Ewa Augustynowicz-Kopec
Agnieszka Iwanska
Pawel Parniewski
Marta Majchrzak
spellingShingle Dariusz Jarych
Ewa Augustynowicz-Kopec
Agnieszka Iwanska
Pawel Parniewski
Marta Majchrzak
Molecular analysis of Pseudomonas aeruginosa strains isolated from cystic fibrosis patients
Scientific Reports
author_facet Dariusz Jarych
Ewa Augustynowicz-Kopec
Agnieszka Iwanska
Pawel Parniewski
Marta Majchrzak
author_sort Dariusz Jarych
title Molecular analysis of Pseudomonas aeruginosa strains isolated from cystic fibrosis patients
title_short Molecular analysis of Pseudomonas aeruginosa strains isolated from cystic fibrosis patients
title_full Molecular analysis of Pseudomonas aeruginosa strains isolated from cystic fibrosis patients
title_fullStr Molecular analysis of Pseudomonas aeruginosa strains isolated from cystic fibrosis patients
title_full_unstemmed Molecular analysis of Pseudomonas aeruginosa strains isolated from cystic fibrosis patients
title_sort molecular analysis of pseudomonas aeruginosa strains isolated from cystic fibrosis patients
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2021-07-01
description Abstract Pseudomonas aeruginosa is a severe bacterial pathogen. Due to the genetic flexibility among strains, chronic airways infection can lead to mortality among cystic fibrosis (CF) patients. It is essential to develop patient-specific therapy which will rely on phenotypic and genomic diversity. The primary objective of this study was to assess the genomic variability of P. aeruginosa strains, using two different molecular techniques for tracking the epidemiological transmissions. This study applied a multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) for an efficient genotyping of clinical P. aeruginosa strains isolated from CF patients and compared results with a TRS-PCR typing. The percentage similarity analysis was performed using the categorical multi-state coefficient and UPGMA method. Based on the MLVA and TRS-PCR group assessment, 43 P. aeruginosa strains/variants were detected among the 63 clinical isolates from eight CF patients. The study of P. aeruginosa isolates has revealed that during chronic bacterial infections, CF patients harbor different P. aeruginosa strains or variants within the same host over the years. P. aeruginosa genotypes diversity may result from infection with several strains and result from a microevolution process of an initially acquired strain. The TRS-PCR method proposed in this work can complement the MLVA scheme. It can also be used as a preliminary method for genetic typing of P. aeruginosa isolates in CF patients.
url https://doi.org/10.1038/s41598-021-95034-2
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