Viricidal activity of several disinfectants against African swine fever virus

Prevention of African swine fever, a disease caused by African swine fever virus (ASFV), requires maintenance of high biosecurity standards, which principally relies on disinfection. Finding the perfect disinfectant against ASFV is difficult because of the lack of relevant data. Therefore, we aimed...

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Main Authors: Cheng-gang JIANG, Ying SUN, Fan ZHANG, Xin AI, Xiao-ning FENG, Wei HU, Xian-feng ZHANG, Dong-ming ZHAO, Zhi-gao BU, Xi-jun HE
Format: Article
Language:English
Published: Elsevier 2021-11-01
Series:Journal of Integrative Agriculture
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2095311921636316
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spelling doaj-05d2443b46214cf0ad2afe2d03c92c102021-09-19T04:56:28ZengElsevierJournal of Integrative Agriculture2095-31192021-11-01201130843088Viricidal activity of several disinfectants against African swine fever virusCheng-gang JIANG0Ying SUN1Fan ZHANG2Xin AI3Xiao-ning FENG4Wei HU5Xian-feng ZHANG6Dong-ming ZHAO7Zhi-gao BU8Xi-jun HE9State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.China; National High Containment Laboratory for Animal Diseases Control and Prevention, Harbin 150069, P.R.ChinaNational High Containment Laboratory for Animal Diseases Control and Prevention, Harbin 150069, P.R.ChinaNational High Containment Laboratory for Animal Diseases Control and Prevention, Harbin 150069, P.R.ChinaNational High Containment Laboratory for Animal Diseases Control and Prevention, Harbin 150069, P.R.ChinaNational High Containment Laboratory for Animal Diseases Control and Prevention, Harbin 150069, P.R.ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.China; National High Containment Laboratory for Animal Diseases Control and Prevention, Harbin 150069, P.R.ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.China; Correspondence ZHAO Dong-ming, Tel: +86-451-51051815State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.China; National High Containment Laboratory for Animal Diseases Control and Prevention, Harbin 150069, P.R.China; Correspondence BU Zhi-gao, Tel: +86-451-51667199State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.China; National High Containment Laboratory for Animal Diseases Control and Prevention, Harbin 150069, P.R.China; Correspondence HE Xi-jun, Tel: +86-451-51051781Prevention of African swine fever, a disease caused by African swine fever virus (ASFV), requires maintenance of high biosecurity standards, which principally relies on disinfection. Finding the perfect disinfectant against ASFV is difficult because of the lack of relevant data. Therefore, we aimed to find the most effective disinfectant and to optimise its concentration as well as contact time to confirm the viricidal effect against ASFV in vitro. We evaluated the viricidal activity of three concentrations each of six common disinfectants against ASFV using immersion disinfection assay (IDA) and spray disinfection assay (SDA); the concentrations of these disinfectants at which complete viral inactivation occurred were almost same as the manufacturer-recommended concentrations, but the exposure times for viral inactivation are different. The following disinfectants (assay: concentration, exposure time) showed complete inactivation: iodine and acid mixed solution (IDA/SDA: 0.5%, 10 min); compound potassium peroxymonosulfate (IDA: 0.25%, 30 min; SDA: 0.25%, 60 min); citric acid (IDA: 0.25%, 60 min; SDA: 0.5%, 60 min); sodium dichloroisocyanurate (IDA: 0.125%, 60 min; SDA: 0.25%, 60 min); and glutaral ang deciquam (IDA/SDA: 0.2%, 60 min); and deciquam (IDA/SDA: 0.5%, 60 min). However, in the presence of organic material contamination, disinfectants did not show a marked inactivation effect. Therefore, disinfection procedures should be performed in two steps: thorough mechanical cleaning followed by application of disinfectant. In conclusion, all the tested disinfectants can inactivate ASFV; these can be used as alternative disinfectants to enhance biosecurity.http://www.sciencedirect.com/science/article/pii/S2095311921636316disinfectionAfrican swine fever virus (ASFV)virucidal effectsbiosecurity
collection DOAJ
language English
format Article
sources DOAJ
author Cheng-gang JIANG
Ying SUN
Fan ZHANG
Xin AI
Xiao-ning FENG
Wei HU
Xian-feng ZHANG
Dong-ming ZHAO
Zhi-gao BU
Xi-jun HE
spellingShingle Cheng-gang JIANG
Ying SUN
Fan ZHANG
Xin AI
Xiao-ning FENG
Wei HU
Xian-feng ZHANG
Dong-ming ZHAO
Zhi-gao BU
Xi-jun HE
Viricidal activity of several disinfectants against African swine fever virus
Journal of Integrative Agriculture
disinfection
African swine fever virus (ASFV)
virucidal effects
biosecurity
author_facet Cheng-gang JIANG
Ying SUN
Fan ZHANG
Xin AI
Xiao-ning FENG
Wei HU
Xian-feng ZHANG
Dong-ming ZHAO
Zhi-gao BU
Xi-jun HE
author_sort Cheng-gang JIANG
title Viricidal activity of several disinfectants against African swine fever virus
title_short Viricidal activity of several disinfectants against African swine fever virus
title_full Viricidal activity of several disinfectants against African swine fever virus
title_fullStr Viricidal activity of several disinfectants against African swine fever virus
title_full_unstemmed Viricidal activity of several disinfectants against African swine fever virus
title_sort viricidal activity of several disinfectants against african swine fever virus
publisher Elsevier
series Journal of Integrative Agriculture
issn 2095-3119
publishDate 2021-11-01
description Prevention of African swine fever, a disease caused by African swine fever virus (ASFV), requires maintenance of high biosecurity standards, which principally relies on disinfection. Finding the perfect disinfectant against ASFV is difficult because of the lack of relevant data. Therefore, we aimed to find the most effective disinfectant and to optimise its concentration as well as contact time to confirm the viricidal effect against ASFV in vitro. We evaluated the viricidal activity of three concentrations each of six common disinfectants against ASFV using immersion disinfection assay (IDA) and spray disinfection assay (SDA); the concentrations of these disinfectants at which complete viral inactivation occurred were almost same as the manufacturer-recommended concentrations, but the exposure times for viral inactivation are different. The following disinfectants (assay: concentration, exposure time) showed complete inactivation: iodine and acid mixed solution (IDA/SDA: 0.5%, 10 min); compound potassium peroxymonosulfate (IDA: 0.25%, 30 min; SDA: 0.25%, 60 min); citric acid (IDA: 0.25%, 60 min; SDA: 0.5%, 60 min); sodium dichloroisocyanurate (IDA: 0.125%, 60 min; SDA: 0.25%, 60 min); and glutaral ang deciquam (IDA/SDA: 0.2%, 60 min); and deciquam (IDA/SDA: 0.5%, 60 min). However, in the presence of organic material contamination, disinfectants did not show a marked inactivation effect. Therefore, disinfection procedures should be performed in two steps: thorough mechanical cleaning followed by application of disinfectant. In conclusion, all the tested disinfectants can inactivate ASFV; these can be used as alternative disinfectants to enhance biosecurity.
topic disinfection
African swine fever virus (ASFV)
virucidal effects
biosecurity
url http://www.sciencedirect.com/science/article/pii/S2095311921636316
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