Isolation of pure LpB from human serum.

Isolation of pure LpB from human serum.

Low density lipoproteins (LDL), even after isolation from a narrow density cut and after several washes by preparative ultracentrifugation, are contaminated by 3-5% non-apoB proteins. Incubation of these LDL with artificial triglyceride-rich lipid emulsions (TGRP) removed all contaminating apoC and...

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Main Authors: R Zechner, R Moser, G M Kostner
Format: Article
Language:English
Published: Elsevier 1988-08-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520388076
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spelling doaj-05cf2e7530464353aeb5725587c538f32021-04-25T04:21:10ZengElsevierJournal of Lipid Research0022-22751988-08-01276681686Isolation of pure LpB from human serum.R ZechnerR MoserG M KostnerLow density lipoproteins (LDL), even after isolation from a narrow density cut and after several washes by preparative ultracentrifugation, are contaminated by 3-5% non-apoB proteins. Incubation of these LDL with artificial triglyceride-rich lipid emulsions (TGRP) removed all contaminating apoC and also, under certain conditions, apoA proteins. TGRP treatment did not, however, change the lipid composition and the flotation behavior of LDL. Residual apoE and albumin, amounting up to 0.5% of the apoB mass, were resistant to removal by TGRP treatment as well as by heparin-Sepharose column chromatography. ApoE and albumin could only be removed by immunoabsorption.http://www.sciencedirect.com/science/article/pii/S0022227520388076
collection DOAJ
language English
format Article
sources DOAJ
author R Zechner
R Moser
G M Kostner
spellingShingle R Zechner
R Moser
G M Kostner
Isolation of pure LpB from human serum.
Journal of Lipid Research
author_facet R Zechner
R Moser
G M Kostner
author_sort R Zechner
title Isolation of pure LpB from human serum.
title_short Isolation of pure LpB from human serum.
title_full Isolation of pure LpB from human serum.
title_fullStr Isolation of pure LpB from human serum.
title_full_unstemmed Isolation of pure LpB from human serum.
title_sort isolation of pure lpb from human serum.
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1988-08-01
description Low density lipoproteins (LDL), even after isolation from a narrow density cut and after several washes by preparative ultracentrifugation, are contaminated by 3-5% non-apoB proteins. Incubation of these LDL with artificial triglyceride-rich lipid emulsions (TGRP) removed all contaminating apoC and also, under certain conditions, apoA proteins. TGRP treatment did not, however, change the lipid composition and the flotation behavior of LDL. Residual apoE and albumin, amounting up to 0.5% of the apoB mass, were resistant to removal by TGRP treatment as well as by heparin-Sepharose column chromatography. ApoE and albumin could only be removed by immunoabsorption.
url http://www.sciencedirect.com/science/article/pii/S0022227520388076
work_keys_str_mv AT rzechner isolationofpurelpbfromhumanserum
AT rmoser isolationofpurelpbfromhumanserum
AT gmkostner isolationofpurelpbfromhumanserum
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