An engineered approach to stem cell culture: automating the decision process for real-time adaptive subculture of stem cells.

Current cell culture practices are dependent upon human operators and remain laborious and highly subjective, resulting in large variations and inconsistent outcomes, especially when using visual assessments of cell confluency to determine the appropriate time to subculture cells. Although efforts t...

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Bibliographic Details
Main Authors: Dai Fei Elmer Ker, Lee E Weiss, Silvina N Junkers, Mei Chen, Zhaozheng Yin, Michael F Sandbothe, Seung-il Huh, Sungeun Eom, Ryoma Bise, Elvira Osuna-Highley, Takeo Kanade, Phil G Campbell
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22110715/?tool=EBI