Application of Symmetry Adapted Function Method for Three-Dimensional Reconstruction of Octahedral Biological Macromolecules
A method for three-dimensional (3D) reconstruction of macromolecule assembles, that is, octahedral symmetrical adapted functions (OSAFs) method, was introduced in this paper and a series of formulations for reconstruction by OSAF method were derived. To verify the feasibility and advantages of the m...
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Series: | International Journal of Biomedical Imaging |
Online Access: | http://dx.doi.org/10.1155/2010/195274 |
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doaj-05b68a69b7db43deb57311c65460469b2020-11-24T20:44:49ZengHindawi LimitedInternational Journal of Biomedical Imaging1687-41881687-41962010-01-01201010.1155/2010/195274195274Application of Symmetry Adapted Function Method for Three-Dimensional Reconstruction of Octahedral Biological MacromoleculesSongjun Zeng0Hongrong Liu1Qibin Yang2Institute of Modern Physics, Xiangtan University, Xiangtan 411105, ChinaInstitute of Modern Physics, Xiangtan University, Xiangtan 411105, ChinaInstitute of Modern Physics, Xiangtan University, Xiangtan 411105, ChinaA method for three-dimensional (3D) reconstruction of macromolecule assembles, that is, octahedral symmetrical adapted functions (OSAFs) method, was introduced in this paper and a series of formulations for reconstruction by OSAF method were derived. To verify the feasibility and advantages of the method, two octahedral symmetrical macromolecules, that is, heat shock protein Degp24 and the Red-cell L Ferritin, were utilized as examples to implement reconstruction by the OSAF method. The schedule for simulation was designed as follows: 2000 random orientated projections of single particles with predefined Euler angles and centers of origins were generated, then different levels of noises that is signal-to-noise ratio (S/N) =0.1,0.5, and 0.8 were added. The structures reconstructed by the OSAF method were in good agreement with the standard models and the relative errors of the structures reconstructed by the OSAF method to standard structures were very little even for high level noise. The facts mentioned above account for that the OSAF method is feasible and efficient approach to reconstruct structures of macromolecules and have ability to suppress the influence of noise.http://dx.doi.org/10.1155/2010/195274 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Songjun Zeng Hongrong Liu Qibin Yang |
spellingShingle |
Songjun Zeng Hongrong Liu Qibin Yang Application of Symmetry Adapted Function Method for Three-Dimensional Reconstruction of Octahedral Biological Macromolecules International Journal of Biomedical Imaging |
author_facet |
Songjun Zeng Hongrong Liu Qibin Yang |
author_sort |
Songjun Zeng |
title |
Application of Symmetry Adapted Function Method for Three-Dimensional Reconstruction of Octahedral Biological Macromolecules |
title_short |
Application of Symmetry Adapted Function Method for Three-Dimensional Reconstruction of Octahedral Biological Macromolecules |
title_full |
Application of Symmetry Adapted Function Method for Three-Dimensional Reconstruction of Octahedral Biological Macromolecules |
title_fullStr |
Application of Symmetry Adapted Function Method for Three-Dimensional Reconstruction of Octahedral Biological Macromolecules |
title_full_unstemmed |
Application of Symmetry Adapted Function Method for Three-Dimensional Reconstruction of Octahedral Biological Macromolecules |
title_sort |
application of symmetry adapted function method for three-dimensional reconstruction of octahedral biological macromolecules |
publisher |
Hindawi Limited |
series |
International Journal of Biomedical Imaging |
issn |
1687-4188 1687-4196 |
publishDate |
2010-01-01 |
description |
A method for three-dimensional (3D) reconstruction of macromolecule assembles, that is, octahedral symmetrical adapted functions (OSAFs) method, was introduced in this paper and a series of formulations for reconstruction by OSAF method were derived. To verify the feasibility and advantages of the method, two octahedral symmetrical macromolecules, that is, heat shock protein Degp24 and the Red-cell L Ferritin, were utilized as examples to implement reconstruction by the OSAF method. The schedule for simulation was designed as follows: 2000 random orientated projections of single particles with predefined Euler angles and centers of origins were generated, then different levels of noises that is signal-to-noise ratio (S/N) =0.1,0.5, and 0.8 were added. The structures reconstructed by the OSAF method were in good agreement with the standard models and the relative errors of the structures reconstructed by the OSAF method to standard structures were very little even for high level noise. The facts mentioned above account for that the OSAF method is feasible and efficient approach to reconstruct structures of macromolecules and have ability to suppress the influence of noise. |
url |
http://dx.doi.org/10.1155/2010/195274 |
work_keys_str_mv |
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1716816502310567936 |