CD34+ cells cultured in stem cell factor and interleukin-2 generate CD56+ cells with antiproliferative effects on tumor cell lines

CD34+ cells cultured in stem cell factor and interleukin-2 generate CD56+ cells with antiproliferative effects on tumor cell lines

<p>Abstract</p> <p>In vitro stimulation of CD34+ cells with IL-2 induces NK cell differentiation. In order to define the stages of NK cell development, which influence their generation from CD34 cells, we cultured G-CSF mobilized peripheral blood CD34+ cells in the presence of stem...

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Bibliographic Details
Main Authors: Hensel Nancy, Li Jongming, Melenhorst Jos, Rezvani Katayoun, Provenzano Maurizio, Sconocchia Giuseppe, Barrett A John
Format: Article
Language:English
Published: BMC 2005-04-01
Series:Journal of Translational Medicine
Subjects:
NK cells
cellular proliferation
cellular differentiation
Online Access:http://www.translational-medicine.com/content/3/1/15
Description
Summary:<p>Abstract</p> <p>In vitro stimulation of CD34+ cells with IL-2 induces NK cell differentiation. In order to define the stages of NK cell development, which influence their generation from CD34 cells, we cultured G-CSF mobilized peripheral blood CD34+ cells in the presence of stem cell factor and IL-2. After three weeks culture we found a diversity of CD56+ subsets which possessed granzyme A, but lacked the cytotoxic apparatus required for classical NK-like cytotoxicity. However, these CD56+ cells had the unusual property of inhibiting proliferation of K562 and P815 cell lines in a cell-contact dependent fashion.</p>
ISSN:1479-5876

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