Fast In Vitro Procedure to Identify Extraembryonic Differentiation Defect of Mouse Embryonic Stem Cells

Fast In Vitro Procedure to Identify Extraembryonic Differentiation Defect of Mouse Embryonic Stem Cells

Summary: Mouse embryonic stem cells (mESCs) are a powerful model to study early mouse development. These blastocyst-derived cells can maintain pluripotency and differentiate into the three embryonic germ layers and an extraembryonic layer, the extraembryonic endoderm (ExEn), which shares similar mol...

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Main Authors: Richard Patryk Ngondo, Michel Cohen-Tannoudji, Constance Ciaudo
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720301143
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spelling doaj-058268f1c9b940eb8cd7f25f47ca58812020-12-21T04:48:47ZengElsevierSTAR Protocols2666-16672020-12-0113100127Fast In Vitro Procedure to Identify Extraembryonic Differentiation Defect of Mouse Embryonic Stem CellsRichard Patryk Ngondo0Michel Cohen-Tannoudji1Constance Ciaudo2University of Strasbourg, Strasbourg, France; Institut de Biologie Moléculaire des Plantes UPR-CNRS 2357, 12 rue du Général Zimmer, 67084 Strasbourg, France; Corresponding authorEarly Mammalian Development and Stem Cell Biology, Institut Pasteur, CNRS UMR 3738, 25 rue du docteur Roux, 75015 Paris, FranceSwiss Federal Institute of Technology Zurich, IMHS, Chair of RNAi and Genome Integrity, 8093 Zurich, Switzerland; Corresponding authorSummary: Mouse embryonic stem cells (mESCs) are a powerful model to study early mouse development. These blastocyst-derived cells can maintain pluripotency and differentiate into the three embryonic germ layers and an extraembryonic layer, the extraembryonic endoderm (ExEn), which shares similar molecular markers to the definitive endoderm. Here, we present a fast procedure to identify a differentiation defect of mESCs toward ExEn in vitro through the molecular and cellular characterization of embryoid bodies, followed by direct differentiation of mESCs into ExEn.For complete details on the use and execution of this protocol, please refer to Ngondo et al. (2018).http://www.sciencedirect.com/science/article/pii/S2666166720301143
collection DOAJ
language English
format Article
sources DOAJ
author Richard Patryk Ngondo
Michel Cohen-Tannoudji
Constance Ciaudo
spellingShingle Richard Patryk Ngondo
Michel Cohen-Tannoudji
Constance Ciaudo
Fast In Vitro Procedure to Identify Extraembryonic Differentiation Defect of Mouse Embryonic Stem Cells
STAR Protocols
author_facet Richard Patryk Ngondo
Michel Cohen-Tannoudji
Constance Ciaudo
author_sort Richard Patryk Ngondo
title Fast In Vitro Procedure to Identify Extraembryonic Differentiation Defect of Mouse Embryonic Stem Cells
title_short Fast In Vitro Procedure to Identify Extraembryonic Differentiation Defect of Mouse Embryonic Stem Cells
title_full Fast In Vitro Procedure to Identify Extraembryonic Differentiation Defect of Mouse Embryonic Stem Cells
title_fullStr Fast In Vitro Procedure to Identify Extraembryonic Differentiation Defect of Mouse Embryonic Stem Cells
title_full_unstemmed Fast In Vitro Procedure to Identify Extraembryonic Differentiation Defect of Mouse Embryonic Stem Cells
title_sort fast in vitro procedure to identify extraembryonic differentiation defect of mouse embryonic stem cells
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2020-12-01
description Summary: Mouse embryonic stem cells (mESCs) are a powerful model to study early mouse development. These blastocyst-derived cells can maintain pluripotency and differentiate into the three embryonic germ layers and an extraembryonic layer, the extraembryonic endoderm (ExEn), which shares similar molecular markers to the definitive endoderm. Here, we present a fast procedure to identify a differentiation defect of mESCs toward ExEn in vitro through the molecular and cellular characterization of embryoid bodies, followed by direct differentiation of mESCs into ExEn.For complete details on the use and execution of this protocol, please refer to Ngondo et al. (2018).
url http://www.sciencedirect.com/science/article/pii/S2666166720301143
work_keys_str_mv AT richardpatrykngondo fastinvitroproceduretoidentifyextraembryonicdifferentiationdefectofmouseembryonicstemcells
AT michelcohentannoudji fastinvitroproceduretoidentifyextraembryonicdifferentiationdefectofmouseembryonicstemcells
AT constanceciaudo fastinvitroproceduretoidentifyextraembryonicdifferentiationdefectofmouseembryonicstemcells
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