Molecular analysis of an integrative conjugative element, ICEH, present in the chromosome of different strains of Mycoplasma hyopneumoniae

Diversification of bacterial species and pathotypes is largely caused by lateral gene transfer (LGT) of diverse mobile DNA elements such as plasmids, phages, transposons and genomic islands. Thus, acquisition of new phenotypes by LGT is very important for bacterial evolution and relationship with ho...

Full description

Bibliographic Details
Main Authors: Paulo Marcos Pinto, Marcos Oliveira de Carvalho, Leonardo Alves-Junior, Marcelo Brocchi, Irene Silveira Schrank
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2007-01-01
Series:Genetics and Molecular Biology
Subjects:
LGT
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200014
id doaj-0563a226c6b54c7c8f90482f3f8d742a
record_format Article
spelling doaj-0563a226c6b54c7c8f90482f3f8d742a2020-11-25T01:37:04ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852007-01-0130125626310.1590/S1415-47572007000200014Molecular analysis of an integrative conjugative element, ICEH, present in the chromosome of different strains of Mycoplasma hyopneumoniaePaulo Marcos PintoMarcos Oliveira de CarvalhoLeonardo Alves-JuniorMarcelo BrocchiIrene Silveira SchrankDiversification of bacterial species and pathotypes is largely caused by lateral gene transfer (LGT) of diverse mobile DNA elements such as plasmids, phages, transposons and genomic islands. Thus, acquisition of new phenotypes by LGT is very important for bacterial evolution and relationship with hosts. This paper reports a 23 kb region containing fourteen CDSs with similarity to the previous described Integrative Conjugal Element of Mycoplasma fermentans (ICEF). This element, named ICEH, is present as one copy at distinct integration sites in the chromosome of 7448 and 232 pathogenic strains and is absent in the type strain J (non-pathogenic). Notable differences in the nucleotide composition of the insertion sites were detected, and could be correlated to a lack of specificity of the ICEH integrase. Although present in strains of the same organism, the ICEH elements are more divergent than the typical similarity between other chromosomal locus of Mycoplasma hyopneunomiae, suggesting an accelerated evolution of these constins or an ongoing process of degeneration, while maintaining conservation of the tra genes. An extrachromosomal form of this element had been detected in the 7448 strain, suggesting a possible involvement in its mobilization and transference of CDSs to new hosts.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200014LGTMycoplasmaconstins
collection DOAJ
language English
format Article
sources DOAJ
author Paulo Marcos Pinto
Marcos Oliveira de Carvalho
Leonardo Alves-Junior
Marcelo Brocchi
Irene Silveira Schrank
spellingShingle Paulo Marcos Pinto
Marcos Oliveira de Carvalho
Leonardo Alves-Junior
Marcelo Brocchi
Irene Silveira Schrank
Molecular analysis of an integrative conjugative element, ICEH, present in the chromosome of different strains of Mycoplasma hyopneumoniae
Genetics and Molecular Biology
LGT
Mycoplasma
constins
author_facet Paulo Marcos Pinto
Marcos Oliveira de Carvalho
Leonardo Alves-Junior
Marcelo Brocchi
Irene Silveira Schrank
author_sort Paulo Marcos Pinto
title Molecular analysis of an integrative conjugative element, ICEH, present in the chromosome of different strains of Mycoplasma hyopneumoniae
title_short Molecular analysis of an integrative conjugative element, ICEH, present in the chromosome of different strains of Mycoplasma hyopneumoniae
title_full Molecular analysis of an integrative conjugative element, ICEH, present in the chromosome of different strains of Mycoplasma hyopneumoniae
title_fullStr Molecular analysis of an integrative conjugative element, ICEH, present in the chromosome of different strains of Mycoplasma hyopneumoniae
title_full_unstemmed Molecular analysis of an integrative conjugative element, ICEH, present in the chromosome of different strains of Mycoplasma hyopneumoniae
title_sort molecular analysis of an integrative conjugative element, iceh, present in the chromosome of different strains of mycoplasma hyopneumoniae
publisher Sociedade Brasileira de Genética
series Genetics and Molecular Biology
issn 1415-4757
1678-4685
publishDate 2007-01-01
description Diversification of bacterial species and pathotypes is largely caused by lateral gene transfer (LGT) of diverse mobile DNA elements such as plasmids, phages, transposons and genomic islands. Thus, acquisition of new phenotypes by LGT is very important for bacterial evolution and relationship with hosts. This paper reports a 23 kb region containing fourteen CDSs with similarity to the previous described Integrative Conjugal Element of Mycoplasma fermentans (ICEF). This element, named ICEH, is present as one copy at distinct integration sites in the chromosome of 7448 and 232 pathogenic strains and is absent in the type strain J (non-pathogenic). Notable differences in the nucleotide composition of the insertion sites were detected, and could be correlated to a lack of specificity of the ICEH integrase. Although present in strains of the same organism, the ICEH elements are more divergent than the typical similarity between other chromosomal locus of Mycoplasma hyopneunomiae, suggesting an accelerated evolution of these constins or an ongoing process of degeneration, while maintaining conservation of the tra genes. An extrachromosomal form of this element had been detected in the 7448 strain, suggesting a possible involvement in its mobilization and transference of CDSs to new hosts.
topic LGT
Mycoplasma
constins
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200014
work_keys_str_mv AT paulomarcospinto molecularanalysisofanintegrativeconjugativeelementicehpresentinthechromosomeofdifferentstrainsofmycoplasmahyopneumoniae
AT marcosoliveiradecarvalho molecularanalysisofanintegrativeconjugativeelementicehpresentinthechromosomeofdifferentstrainsofmycoplasmahyopneumoniae
AT leonardoalvesjunior molecularanalysisofanintegrativeconjugativeelementicehpresentinthechromosomeofdifferentstrainsofmycoplasmahyopneumoniae
AT marcelobrocchi molecularanalysisofanintegrativeconjugativeelementicehpresentinthechromosomeofdifferentstrainsofmycoplasmahyopneumoniae
AT irenesilveiraschrank molecularanalysisofanintegrativeconjugativeelementicehpresentinthechromosomeofdifferentstrainsofmycoplasmahyopneumoniae
_version_ 1725059793248321536