EFEITO DOS MEIOS DILUÍDORES MMC (MÍNIMA CONTAMINAÇÃO) E LGM (LACTOSE-GEMA MODIFICADO) NA VIABILIDADE DO SÊMEN DE CÃES CONSERVADO POR 24 HORAS EM EQUITAINER®.
The aim of this study was to compare the viability of canine semen after dilution in Minimum Contamination Medium (MMC) and Modified Lactose-Egg yolk extender (LGM) and incubation at 4 ºC for 12 and 24 hours. Thirteen ejaculates were collected from 5 adult dogs by digital manipulation. Macroscopic a...
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Universidade Federal de Goiás
2011-03-01
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doaj-055d35991f1a4fa48181ccbdcb6795132020-11-25T04:04:10ZengUniversidade Federal de GoiásCiência Animal Brasileira1518-27971089-68912011-03-01121126133EFEITO DOS MEIOS DILUÍDORES MMC (MÍNIMA CONTAMINAÇÃO) E LGM (LACTOSE-GEMA MODIFICADO) NA VIABILIDADE DO SÊMEN DE CÃES CONSERVADO POR 24 HORAS EM EQUITAINER®.Tania Goes de PinhoLuis Garcia NogueiraFelipe Zandonadi BrandãoLuciano JamasBianca Perez BarcellosMarco Antônio RochaThe aim of this study was to compare the viability of canine semen after dilution in Minimum Contamination Medium (MMC) and Modified Lactose-Egg yolk extender (LGM) and incubation at 4 ºC for 12 and 24 hours. Thirteen ejaculates were collected from 5 adult dogs by digital manipulation. Macroscopic and microscopic characteristics were assessed right after collection. Semen was divided to be diluted (1:3) in MMC and LGM and subsequently chilled in Equitainer®. Seminal parameters of motility and spermatic morphology, membrane integrity (hyposmotic test), spermatic viability and spontaneous acrosome reaction (Trypan-blue Giemsa stain) were evaluated in fresh and chilled semen after 12 and 24 hours of incubation at 4 ºC. No difference between extenders was identified in semen conservation after 12 hours. After 24 hours just spermatic motility was different (p>0.05). But, after both periods of conservation, semen diluted in LGM maintained most of the characteristics verified in the fresh semen. The mean of true and false acrosome reaction did not exceed 2% in both semen extenders, which demonstrates no influence of media component and incubation period on these phenomena at 4 ºC. In conclusion, these results indicate that both extenders can be used in canine semen conservation at 4 ºC in contêiner during 12 hours without significant changes in semen characteristics.http://www.revistas.ufg.br/index.php/vet/article/view/4692Dogsspermcooling. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tania Goes de Pinho Luis Garcia Nogueira Felipe Zandonadi Brandão Luciano Jamas Bianca Perez Barcellos Marco Antônio Rocha |
spellingShingle |
Tania Goes de Pinho Luis Garcia Nogueira Felipe Zandonadi Brandão Luciano Jamas Bianca Perez Barcellos Marco Antônio Rocha EFEITO DOS MEIOS DILUÍDORES MMC (MÍNIMA CONTAMINAÇÃO) E LGM (LACTOSE-GEMA MODIFICADO) NA VIABILIDADE DO SÊMEN DE CÃES CONSERVADO POR 24 HORAS EM EQUITAINER®. Ciência Animal Brasileira Dogs sperm cooling. |
author_facet |
Tania Goes de Pinho Luis Garcia Nogueira Felipe Zandonadi Brandão Luciano Jamas Bianca Perez Barcellos Marco Antônio Rocha |
author_sort |
Tania Goes de Pinho |
title |
EFEITO DOS MEIOS DILUÍDORES MMC (MÍNIMA CONTAMINAÇÃO) E LGM (LACTOSE-GEMA MODIFICADO) NA VIABILIDADE DO SÊMEN DE CÃES CONSERVADO POR 24 HORAS EM EQUITAINER®. |
title_short |
EFEITO DOS MEIOS DILUÍDORES MMC (MÍNIMA CONTAMINAÇÃO) E LGM (LACTOSE-GEMA MODIFICADO) NA VIABILIDADE DO SÊMEN DE CÃES CONSERVADO POR 24 HORAS EM EQUITAINER®. |
title_full |
EFEITO DOS MEIOS DILUÍDORES MMC (MÍNIMA CONTAMINAÇÃO) E LGM (LACTOSE-GEMA MODIFICADO) NA VIABILIDADE DO SÊMEN DE CÃES CONSERVADO POR 24 HORAS EM EQUITAINER®. |
title_fullStr |
EFEITO DOS MEIOS DILUÍDORES MMC (MÍNIMA CONTAMINAÇÃO) E LGM (LACTOSE-GEMA MODIFICADO) NA VIABILIDADE DO SÊMEN DE CÃES CONSERVADO POR 24 HORAS EM EQUITAINER®. |
title_full_unstemmed |
EFEITO DOS MEIOS DILUÍDORES MMC (MÍNIMA CONTAMINAÇÃO) E LGM (LACTOSE-GEMA MODIFICADO) NA VIABILIDADE DO SÊMEN DE CÃES CONSERVADO POR 24 HORAS EM EQUITAINER®. |
title_sort |
efeito dos meios diluídores mmc (mínima contaminação) e lgm (lactose-gema modificado) na viabilidade do sêmen de cães conservado por 24 horas em equitainer®. |
publisher |
Universidade Federal de Goiás |
series |
Ciência Animal Brasileira |
issn |
1518-2797 1089-6891 |
publishDate |
2011-03-01 |
description |
The aim of this study was to compare the viability of canine semen after dilution in Minimum Contamination Medium (MMC) and Modified Lactose-Egg yolk extender (LGM) and incubation at 4 ºC for 12 and 24 hours. Thirteen ejaculates were collected from 5 adult dogs by digital manipulation. Macroscopic and microscopic characteristics were assessed right after collection. Semen was divided to be diluted (1:3) in MMC and LGM and subsequently chilled in Equitainer®. Seminal parameters of motility and spermatic morphology, membrane integrity (hyposmotic test), spermatic viability and spontaneous acrosome reaction (Trypan-blue Giemsa stain) were evaluated in fresh and chilled semen after 12 and 24 hours of incubation at 4 ºC. No difference between extenders was identified in semen conservation after 12 hours. After 24 hours just spermatic motility was different (p>0.05). But, after both periods of conservation, semen diluted in LGM maintained most of the characteristics verified in the fresh semen. The mean of true and false acrosome reaction did not exceed 2% in both semen extenders, which demonstrates no influence of media component and incubation period on these phenomena at 4 ºC. In conclusion, these results indicate that both extenders can be used in canine semen conservation at 4 ºC in contêiner during 12 hours without significant changes in semen characteristics. |
topic |
Dogs sperm cooling. |
url |
http://www.revistas.ufg.br/index.php/vet/article/view/4692 |
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