EFEITO DOS MEIOS DILUÍDORES MMC (MÍNIMA CONTAMINAÇÃO) E LGM (LACTOSE-GEMA MODIFICADO) NA VIABILIDADE DO SÊMEN DE CÃES CONSERVADO POR 24 HORAS EM EQUITAINER®.

The aim of this study was to compare the viability of canine semen after dilution in Minimum Contamination Medium (MMC) and Modified Lactose-Egg yolk extender (LGM) and incubation at 4 ºC for 12 and 24 hours. Thirteen ejaculates were collected from 5 adult dogs by digital manipulation. Macroscopic a...

Full description

Bibliographic Details
Main Authors: Tania Goes de Pinho, Luis Garcia Nogueira, Felipe Zandonadi Brandão, Luciano Jamas, Bianca Perez Barcellos, Marco Antônio Rocha
Format: Article
Language:English
Published: Universidade Federal de Goiás 2011-03-01
Series:Ciência Animal Brasileira
Subjects:
Online Access:http://www.revistas.ufg.br/index.php/vet/article/view/4692
id doaj-055d35991f1a4fa48181ccbdcb679513
record_format Article
spelling doaj-055d35991f1a4fa48181ccbdcb6795132020-11-25T04:04:10ZengUniversidade Federal de GoiásCiência Animal Brasileira1518-27971089-68912011-03-01121126133EFEITO DOS MEIOS DILUÍDORES MMC (MÍNIMA CONTAMINAÇÃO) E LGM (LACTOSE-GEMA MODIFICADO) NA VIABILIDADE DO SÊMEN DE CÃES CONSERVADO POR 24 HORAS EM EQUITAINER®.Tania Goes de PinhoLuis Garcia NogueiraFelipe Zandonadi BrandãoLuciano JamasBianca Perez BarcellosMarco Antônio RochaThe aim of this study was to compare the viability of canine semen after dilution in Minimum Contamination Medium (MMC) and Modified Lactose-Egg yolk extender (LGM) and incubation at 4 ºC for 12 and 24 hours. Thirteen ejaculates were collected from 5 adult dogs by digital manipulation. Macroscopic and microscopic characteristics were assessed right after collection. Semen was divided to be diluted (1:3) in MMC and LGM and subsequently chilled in Equitainer®. Seminal parameters of motility and spermatic morphology, membrane integrity (hyposmotic test), spermatic viability and spontaneous acrosome reaction (Trypan-blue Giemsa stain) were evaluated in fresh and chilled semen after 12 and 24 hours of incubation at 4 ºC. No difference between extenders was identified in semen conservation after 12 hours. After 24 hours just spermatic motility was different (p>0.05). But, after both periods of conservation, semen diluted in LGM maintained most of the characteristics verified in the fresh semen. The mean of true and false acrosome reaction did not exceed 2% in both semen extenders, which demonstrates no influence of media component and incubation period on these phenomena at 4 ºC. In conclusion, these results indicate that both extenders can be used in canine semen conservation at 4 ºC in contêiner during 12 hours without significant changes in semen characteristics.http://www.revistas.ufg.br/index.php/vet/article/view/4692Dogsspermcooling.
collection DOAJ
language English
format Article
sources DOAJ
author Tania Goes de Pinho
Luis Garcia Nogueira
Felipe Zandonadi Brandão
Luciano Jamas
Bianca Perez Barcellos
Marco Antônio Rocha
spellingShingle Tania Goes de Pinho
Luis Garcia Nogueira
Felipe Zandonadi Brandão
Luciano Jamas
Bianca Perez Barcellos
Marco Antônio Rocha
EFEITO DOS MEIOS DILUÍDORES MMC (MÍNIMA CONTAMINAÇÃO) E LGM (LACTOSE-GEMA MODIFICADO) NA VIABILIDADE DO SÊMEN DE CÃES CONSERVADO POR 24 HORAS EM EQUITAINER®.
Ciência Animal Brasileira
Dogs
sperm
cooling.
author_facet Tania Goes de Pinho
Luis Garcia Nogueira
Felipe Zandonadi Brandão
Luciano Jamas
Bianca Perez Barcellos
Marco Antônio Rocha
author_sort Tania Goes de Pinho
title EFEITO DOS MEIOS DILUÍDORES MMC (MÍNIMA CONTAMINAÇÃO) E LGM (LACTOSE-GEMA MODIFICADO) NA VIABILIDADE DO SÊMEN DE CÃES CONSERVADO POR 24 HORAS EM EQUITAINER®.
title_short EFEITO DOS MEIOS DILUÍDORES MMC (MÍNIMA CONTAMINAÇÃO) E LGM (LACTOSE-GEMA MODIFICADO) NA VIABILIDADE DO SÊMEN DE CÃES CONSERVADO POR 24 HORAS EM EQUITAINER®.
title_full EFEITO DOS MEIOS DILUÍDORES MMC (MÍNIMA CONTAMINAÇÃO) E LGM (LACTOSE-GEMA MODIFICADO) NA VIABILIDADE DO SÊMEN DE CÃES CONSERVADO POR 24 HORAS EM EQUITAINER®.
title_fullStr EFEITO DOS MEIOS DILUÍDORES MMC (MÍNIMA CONTAMINAÇÃO) E LGM (LACTOSE-GEMA MODIFICADO) NA VIABILIDADE DO SÊMEN DE CÃES CONSERVADO POR 24 HORAS EM EQUITAINER®.
title_full_unstemmed EFEITO DOS MEIOS DILUÍDORES MMC (MÍNIMA CONTAMINAÇÃO) E LGM (LACTOSE-GEMA MODIFICADO) NA VIABILIDADE DO SÊMEN DE CÃES CONSERVADO POR 24 HORAS EM EQUITAINER®.
title_sort efeito dos meios diluídores mmc (mínima contaminação) e lgm (lactose-gema modificado) na viabilidade do sêmen de cães conservado por 24 horas em equitainer®.
publisher Universidade Federal de Goiás
series Ciência Animal Brasileira
issn 1518-2797
1089-6891
publishDate 2011-03-01
description The aim of this study was to compare the viability of canine semen after dilution in Minimum Contamination Medium (MMC) and Modified Lactose-Egg yolk extender (LGM) and incubation at 4 ºC for 12 and 24 hours. Thirteen ejaculates were collected from 5 adult dogs by digital manipulation. Macroscopic and microscopic characteristics were assessed right after collection. Semen was divided to be diluted (1:3) in MMC and LGM and subsequently chilled in Equitainer®. Seminal parameters of motility and spermatic morphology, membrane integrity (hyposmotic test), spermatic viability and spontaneous acrosome reaction (Trypan-blue Giemsa stain) were evaluated in fresh and chilled semen after 12 and 24 hours of incubation at 4 ºC. No difference between extenders was identified in semen conservation after 12 hours. After 24 hours just spermatic motility was different (p>0.05). But, after both periods of conservation, semen diluted in LGM maintained most of the characteristics verified in the fresh semen. The mean of true and false acrosome reaction did not exceed 2% in both semen extenders, which demonstrates no influence of media component and incubation period on these phenomena at 4 ºC. In conclusion, these results indicate that both extenders can be used in canine semen conservation at 4 ºC in contêiner during 12 hours without significant changes in semen characteristics.
topic Dogs
sperm
cooling.
url http://www.revistas.ufg.br/index.php/vet/article/view/4692
work_keys_str_mv AT taniagoesdepinho efeitodosmeiosdiluidoresmmcminimacontaminacaoelgmlactosegemamodificadonaviabilidadedosemendecaesconservadopor24horasemequitainer
AT luisgarcianogueira efeitodosmeiosdiluidoresmmcminimacontaminacaoelgmlactosegemamodificadonaviabilidadedosemendecaesconservadopor24horasemequitainer
AT felipezandonadibrandao efeitodosmeiosdiluidoresmmcminimacontaminacaoelgmlactosegemamodificadonaviabilidadedosemendecaesconservadopor24horasemequitainer
AT lucianojamas efeitodosmeiosdiluidoresmmcminimacontaminacaoelgmlactosegemamodificadonaviabilidadedosemendecaesconservadopor24horasemequitainer
AT biancaperezbarcellos efeitodosmeiosdiluidoresmmcminimacontaminacaoelgmlactosegemamodificadonaviabilidadedosemendecaesconservadopor24horasemequitainer
AT marcoantoniorocha efeitodosmeiosdiluidoresmmcminimacontaminacaoelgmlactosegemamodificadonaviabilidadedosemendecaesconservadopor24horasemequitainer
_version_ 1724437464177704960