EFEITO DOS MEIOS DILUÍDORES MMC (MÍNIMA CONTAMINAÇÃO) E LGM (LACTOSE-GEMA MODIFICADO) NA VIABILIDADE DO SÊMEN DE CÃES CONSERVADO POR 24 HORAS EM EQUITAINER®.

• Main Authors: Tania Goes de Pinho, Luis Garcia Nogueira, Felipe Zandonadi Brandão, Luciano Jamas, Bianca Perez Barcellos, Marco Antônio Rocha
• Format: Article
• Language: English
• Published: Universidade Federal de Goiás 2011-03-01
• Series: Ciência Animal Brasileira
• Subjects: Dogs; sperm; cooling.
• Online Access: http://www.revistas.ufg.br/index.php/vet/article/view/4692
• ISSN: 1518-2797; 1089-6891

The aim of this study was to compare the viability of canine semen after dilution in Minimum Contamination Medium (MMC) and Modified Lactose-Egg yolk extender (LGM) and incubation at 4 ºC for 12 and 24 hours. Thirteen ejaculates were collected from 5 adult dogs by digital manipulation. Macroscopic and microscopic characteristics were assessed right after collection. Semen was divided to be diluted (1:3) in MMC and LGM and subsequently chilled in Equitainer®. Seminal parameters of motility and spermatic morphology, membrane integrity (hyposmotic test), spermatic viability and spontaneous acrosome reaction (Trypan-blue Giemsa stain) were evaluated in fresh and chilled semen after 12 and 24 hours of incubation at 4 ºC. No difference between extenders was identified in semen conservation after 12 hours. After 24 hours just spermatic motility was different (p>0.05). But, after both periods of conservation, semen diluted in LGM maintained most of the characteristics verified in the fresh semen. The mean of true and false acrosome reaction did not exceed 2% in both semen extenders, which demonstrates no influence of media component and incubation period on these phenomena at 4 ºC. In conclusion, these results indicate that both extenders can be used in canine semen conservation at 4 ºC in contêiner during 12 hours without significant changes in semen characteristics.